Neurological and Endocrinological analysis of the output pathways of circadian clock driving eclosion rhythm in the flies, Drosophila melanogaster and Sarcophaga crassipalpis

果蝇、果蝇和巨须石蝇生物钟驱动羽化节律的输出途径的神经学和内分泌学分析

• 批准号: 22570076
• 负责人: YASUYAMA Kouji
• 金额: $ 3万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570076/
• 关键词: 羽化リズム; ハエ目昆虫; 概日時計; ペプチドニューロン; 免疫電顕法; キイロショウジョウバエ; シリアカニクバエ; 免疫組織化学; PDF; CCAP

To understand how the circadian clocks time the gate of eclosion in Drosophila melanogaster, we examined the neuronal connection focusing on the putative clock neurons, PDF-Tri neurons, that transiently occur associated with eclosion, and showed the synaptic connections between the PDF-Tri and the peptidergic (CCAP-, EH- or corazonin) neurons involved in eclosion behavior. We also analyzed the eclosion rhythms in the flesh fly Sarcophaga crassipalpis, and revealed that two mechanisms are required for determination of the gate of eclosion. One is driven by low titer of 20-hydroxyecdysone between 20 h and 16 h before eclosion, and the other is driven by the signal from the circadian clock transmitted later than 6 h before eclosion.

为了了解昼夜节律如何时间到果蝇中的Eclosion大门，我们检查了关注推定的时钟神经元的神经元连接，PDF-TRI神经元，这些神经元与ECLOSION瞬时发生，并显示参与ECOLOSION行为的肽吉尼（CCAP-，EH-或Corazonin）神经元。我们还分析了肉蝇果皮crassipalpis中的eClosion节奏，并揭示了确定eClosion门需要的两种机制。一种是由在eClosion前20小时至16小时之间的20小时至16小时之间的低滴度驱动的，另一个是由昼夜节流驱动的，该信号在eclosion前的6小时以前迟到了6小时。

项目成果

Blocking synaptic transmission with tetanus toxin light chain reveals modes of neurotransmission in the PDF-positive circadian clock neurons of Drosophila melanogaster

用破伤风毒素轻链阻断突触传递揭示了果蝇 PDF 阳性生物钟神经元的神经传递模式

• DOI: 10.1016/j.jinsphys.2011.06.004
• 发表时间: 2011
• 期刊: Journal of Insect Physiology
• 影响因子: 2.2
• 作者: Umezaki;Yujiro
• 通讯作者: Yujiro

Morphological analysis of neuronal connections between pigment-dispersing factor-immunoreactive neurons and pars intercerebralis neurons in the blow fly, Protophormia terraenovae.

丽蝇 Protophormia terraenovae 中色素分散因子免疫反应性神经元和脑间部神经元之间神经元连接的形态学分析。

• 发表时间: 2012
• 作者: Hase;Hiroaki
• 通讯作者: Hiroaki

The neuronal circuit underlying timing of eclosion behavior in Drosophila.

果蝇羽化行为定时的神经元回路。

• 发表时间: 2013
• 作者: Selcho M.;Yasuyama K.;Hensgen R.;WegenerC
• 通讯作者: WegenerC

Anatomy of peptidergic neurons involved in Drosophila eclosion

参与果蝇羽化的肽能神经元的解剖

• 发表时间: 2012
• 作者: Selcho M.;Yasuyama K.;Hensgen R.;WegenerC
• 通讯作者: WegenerC

Anatomy of peptidergic neurons involved in Drosophila eclosion.

参与果蝇羽化的肽能神经元的解剖。

• 发表时间: 2012
• 作者: Selcho;Mareike
• 通讯作者: Mareike