Structural bases for an expanded codon recognition by modified nucleotide.

通过修饰核苷酸扩展密码子识别的结构基础。

• 批准号: 22510242
• 负责人: TAKEMOTO Chi
• 金额: $ 2.91万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22510242/
• 关键词: リボソーム; X線結晶構造解析; 超分子複合; tRNA; 修飾塩基; コドン認識; Lysidine; 結晶構造解析; tRA Ile2; タンパク質合成

Some modified nucleotides in the first letter of a tRNA anticodon is known to expand the codon recognition ability. I planed to reveal the mechanism based on the three-dimensional structure of the codon-anticodon basepair in the 70S ribosome.In this study, I have succeeded to establish a reproducible method of 70S ribosome preparation for highly diffracted crystals. Once good crystals are obtained, technical improvements are required in the following steps: refinement of crystallization condition, less damage for crystals during harvesting, and strategy of data collection at an atomic resolution.

已知tRNA反密码子的第一个字母中的一些修饰核苷酸已知可以扩大密码子识别能力。我计划根据70年代的核糖体揭示基于密码子 - 抗原基台基的三维结构的机制。在这项研究中，我成功地建立了一种可再现的70S核糖体准备方法，以进行高度衍射的晶体。一旦获得了良好的晶体，就需要在以下步骤中进行技术改进：结晶条件的细化，收获过程中晶体的损害较小，以及以原子分辨率收集数据的策略。