Development of rapid biomarker measurement system for functional analysis of human skin

开发用于人体皮肤功能分析的快速生物标志物测量系统

• 批准号: 22500434
• 负责人: YOSHIDA Satoshi
• 金额: $ 3万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500434/
• 关键词: 検査診断システム; バイオマーカー; 赤外分析; 口唇; 糖尿病; 多変量解析; 糖化最終産物; HbA1c; カルボキシメチル化; 非侵襲的; 皮膚; 血糖値; 多奕量解析

I have investigated to develop a non-invasive/non-destructive and instantaneous measurement system of human skin (Skin Function Analysis) as a measurable site of physiological marker materials including fatty acids, glycated end-products and many biomolecules appeared on the skin surface. As a consequence, I have obtained mainly three important results:(1) By loading certain amount of magnesium lactate (Mg-lac) on arm skin surface, the amount of skin surface histamine synthesized was significantly decreased. Mg-lac which appears continuously on the skin surface seems to function to control the histamine synthesis in the skin.(2) Dietary fatty acid, DHA (docosahexaenoic acid), could be observed by FTIR to appear on the lip surface after 1~2 hours later. The appearance profile of DHA on the lip surface was different between males and females, and the significant appearance was observed more clearly for males than females(3) The prediction of HbA1c values for diabetes patients became possible by measuring with FTIR directly the glycated end-products (such as carboxymethylated (CM) arginine and phosphatidyl- ethanolamine (PE)) on the human lip vermillion surface, using PLSregression method.

我已经研究了为人类皮肤（皮肤功能分析）的非侵入性/非毁灭性和瞬时测量系统（作为生理标记物材料的可测量位点），包括脂肪酸，脱糖终产物和许多生物分子出现在皮肤表面上。结果，我主要获得了三个重要的结果：（1）通过将一定量的乳酸镁（MG-LAC）加载到手臂皮肤表面上，可以显着降低皮肤表面组胺的量。 MG-LAC在皮肤表面连续出现似乎可以控制皮肤中的组胺合成。（2）FTIR可以观察到饮食中的脂肪酸DHA（Docosahecahecahexaenoic Acid），在1〜2小时后，FTIR可以出现在唇部表面上。 The appearance profile of DHA on the lip surface was different between males and females, and the significant appearance was observed more clearly for males than females(3) The prediction of HbA1c values for diabetes patients became possible by measuring with FTIR directly the glycated end-products (such as carboxymethylated (CM) arginine and phosphatidyl- ethanolamine (PE)) on the human lip vermillion surface,使用plsrecression方法。

项目成果

Development of noninvasive and mobile FTIR techniques for measurement of human body metabolism.

开发用于测量人体新陈代谢的无创和移动 FTIR 技术。

• 发表时间: 2010
• 作者: Reiko Mizuno;Shinichi Fujimoto;Yoshihiko Saito;Yasuyuki Okamoto;Satoshi Yoshida (招待)
• 通讯作者: Satoshi Yoshida (招待)

Advances in Planar Lipid Bilayers and Liposomes (Chapter 1 : Lipid and membrane dynamics in biological tissues-Infrared spectroscopic studies.)

平面脂质双层和脂质体的进展（第 1 章：生物组织中的脂质和膜动力学 - 红外光谱研究。）

• 发表时间: 2011
• 作者: Satoshi Yoshida;Kenzo Koike (章分担執筆)
• 通讯作者: Kenzo Koike (章分担執筆)

Optical Screening of Diabetes Mellitus using Non-invasive Fourier-transform Infrared Spectroscopy Technique for Human Lip.

使用非侵入性傅里叶变换红外光谱技术对人唇进行糖尿病光学筛查。

• DOI: 10.1016/j.jpba.2012.12.009
• 发表时间: 2013
• 期刊: J. Pharm. Biomed. Anal.
• 作者: Yoshida;S.
• 通讯作者: S.

in2010 International Chemical Congress of Pacific Basin Societies(Honolulu, Hawaii)

2010年太平洋盆地学会国际化学大会（夏威夷檀香山）

• 发表时间: 2012
• 作者: 林修司;山田博胤;Yo shi da S.
• 通讯作者: Yo shi da S.

脂質・脂肪酸アセスメントにおける非破壊的計測法の課題

脂质和脂肪酸评估中无损测量方法的问题

• 发表时间: 2011
• 期刊: 脂質栄養学
• 作者: 佐藤健二;萩原啓;吉田敏
• 通讯作者: 吉田敏