CXCL14/BRAK is a multifunctional tumor suppressor

CXCL14/BRAK是一种多功能肿瘤抑制因子

• 批准号: 22390353
• 负责人: HATA Ryu-ichiro
• 金额: $ 12.73万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390353/
• 关键词: 癌抑制因子; ケモカイン; 転移抑制; 副作用のない癌抑制法; 腫瘍抑制; CXCL14/BRAK; BRAK; CXCL14; ドーマントセラピー; 血管新生阻害; NK細胞

Objectives: The aim of this study was to determine whether or not chemokine BRAK/ CXCL14 transgenic (Tg) mice would show resistance to carcinogenesis, tumor growth and tumor metastasis. Methods: Wild type C57BL/6 (Wt) or Tg mice were injected intraperitoneally with 12 mg/kg body weight azoxymethane followed by intermittent addition of 3 % dextran sodium sulfate in the drinking water. We also injected tumor cells subcutaneously or via a tail vein of the mice. Results: No difference of body weight was observed between Wt and Tg mice but the rate of colorectal cancer was nearly ten times higher (P<0.001) in Wt mice than in Tg ones. Size of transplanted tumors and the number of metastatic foci on the lung of the animals were always significantly lower in the Tg that those in the Wt. Survival rates of the melanoma cell injected Tg mice were significantly higher than those of Wt. Conclusion: The Tg mice showed resistance to carcinogenesis, tumor growth and tumor cell metastasis, indicating BRAK/ CXCL14 has multifunctional effects on tumor suppression.

目的：本研究的目的是确定趋化因子 BRAK/CXCL14 转基因（Tg）小鼠是否表现出对癌发生、肿瘤生长和肿瘤转移的抵抗力。方法：野生型C57BL/6（Wt）或Tg小鼠腹腔注射12 mg/kg体重的氧化偶氮甲烷，随后在饮用水中间歇性添加3%葡聚糖硫酸钠。我们还皮下或通过小鼠尾静脉注射肿瘤细胞。结果：Wt和Tg小鼠体重无差异，但Wt小鼠结直肠癌发生率比Tg小鼠高近10倍（P<0.001）。 Tg 组中移植肿瘤的大小和肺上转移灶的数量始终显着低于 Wt 组。注射黑色素瘤细胞的Tg小鼠的存活率显着高于Wt小鼠。结论：Tg小鼠表现出抗癌、抗肿瘤生长和肿瘤细胞转移的能力，表明BRAK/CXCL14具有抑制肿瘤的多功能作用。

项目成果

Oxidative stress reduces expression of CXCL14/BRAK in human SCC cells

氧化应激降低人鳞状细胞癌细胞中 CXCL14/BRAK 的表达

• 发表时间: 2011
• 作者: Maehata Y;Ozawa S;Miyamoto C;Kobayashi K;Komori R;Hata R;Lee M
• 通讯作者: Lee M

ROCK阻害剤(Fasudi1)は抗腫瘍性ケモカイン(CXCL14/BRAK)の細胞外分泌促進を介して腫瘍進展を抑制する

ROCK 抑制剂 (Fasudi1) 通过促进抗肿瘤趋化因子 (CXCL14/BRAK) 的细胞外分泌来抑制肿瘤进展

• 发表时间: 2011
• 作者: 宮本千央;前畑洋次郎;小澤重幸;生駒丈晴;小森令賀;居作和人;加藤靖正;畑隆一郎;李昌一
• 通讯作者: 李昌一

Fasudil, a specific inhibitor of ROCK, stimulates secretion of CXCL14/BRAK and suppresses tumor growth in vivo

Fasudil 是 ROCK 的特异性抑制剂，可刺激 CXCL14/BRAK 的分泌并抑制体内肿瘤生长

• 发表时间: 2011
• 作者: Miyamoto C.;Maehata Y.;Ozawa S.;Ikoma T.;Komori R.;Hata R.-I.;Lee M-C.
• 通讯作者: Lee M-C.

Reactive oxygen species (ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells

• DOI: 10.3109/10715762.2010.490836
• 发表时间: 2010-08-01
• 期刊: FREE RADICAL RESEARCH
• 影响因子: 3.3
• 作者: Maehata, Yojiro;Ozawa, Shigeyuki;Lee, Masaichi-Chang-Il
• 通讯作者: Lee, Masaichi-Chang-Il

Lee M-C: ROCK Inhibitor Fasudil suppresses growth of fibrosarcoma by stimulating secretion of chemokine

Lee M-C：ROCK抑制剂法舒地尔通过刺激趋化因子的分泌抑制纤维肉瘤的生长

• 发表时间: 2012
• 作者: Miyamoto C.;Maehata Y;Ozawa S.,Ikoma T.;Hata R.
• 通讯作者: Hata R.