Regulation system of differentiation from mesenchymal stem cells to bone, vascular, and fatty tissues by mechanical loading and unloading

间充质干细胞通过机械加载和卸载分化为骨、血管和脂肪组织的调控系统

• 批准号: 22390295
• 负责人: SAKAI Akinori
• 金额: $ 5.32万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390295/
• 关键词: 荷重; 非荷重; 骨芽細胞; 血管内皮細胞; 脂肪細胞; 未分化間葉系幹細胞; 骨髄細胞; 副甲状腺ホルモン受容体; 海綿骨; 皮質骨; p21; PTH/PTHrP受容体; アルデヒド脱水素酵素; 骨膜; 副甲状腺ホルモン; CFU-f; オイルレッドO; 骨形成; CD38; CD31; 間葉系幹細胞; wnt; PECAM-1; 荷重; 非荷重; 骨芽細胞; 血管内皮細胞; 脂肪細胞; 未分化間葉系幹細胞; 骨髄細胞; 副甲状腺ホルモン受容体; 海綿骨; 皮質骨; p21; PTH/PTHrP受容体; アルデヒド脱水素酵素; 骨膜; 副甲状腺ホルモン; CFU-f; オイルレッドO; 骨形成; CD38; CD31; 間葉系幹細胞; wnt; PECAM-1

We performed studies using exercise loading mice and unloading mice due to tail suspension. We demonstrated that the signal through PTH/PTHrP receptors promoted osteogenic differentiation and suppressed adipogenic differentiation from mesenchyman stem cells under loading condition. This signal is necessary for promotion of periosteal bone formation and enlargement of cross-sectional cortical shell. The expressions of CD31 and its receptor CD38 decreased after unloading and recovered to the normal loading levels after reloading. Co-culture experiments of osteoblasts and CD38 positive cells revealed that vascular endothelial factors are necessary for osteogenic differentiation.

我们使用锻炼的小鼠进行了研究，并由于尾悬浮而卸下小鼠。我们证明，通过PTH/PTHRP受体的信号促进了成骨分化，并抑制了在负载条件下与中等基因人干细胞的掺杂分化。该信号对于促进骨膜骨形成和横截面皮质壳的扩大是必需的。卸载后CD31及其受体CD38的表达降低，并在重新加载后恢复到正常负载水平。成骨细胞和CD38阳性细胞的共培养实验表明，血管内皮因子对于成骨分化是必需的。