Molecular pathological analyses of collateral augmentation for a development of novel angiogenic therapy

侧枝增强的分子病理学分析，用于开发新型血管生成疗法

• 批准号: 22390245
• 负责人: MIYATA Tetsurou
• 金额: $ 10.32万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390245/
• 关键词: 血管外科学; 再生医療; 側副血行路; 血管新生療法; ラビット虚血肢モデル; 塩基性線維芽細胞増殖因子

In the present study, we first assessed a process of collateral augmentation in the rabbit model of limb ischemia. After an excision of the left femoral artery, main route of collateral vessels was developed between the left inferior gluteal artery and the left popliteal artery, which was located in the left coccygeo-femoral muscle. Based on the findings, we selectively administrated basic fibroblast growth factor (bFGF) or bone marrow mononuclear cells (MN) to the left coccygeo-femoral muscle or the left adductor muscle at 28 days after femoral artery removal. At 28 days after administration of bFGF or MN, limb ischemia after the delivery to the coccygeo-femoral muscle were significantly improved as compared with those to the adductor muscle, suggesting that selection of delivery target was an important factor to promote effective collateral augmentation.

在本研究中，我们首先评估了兔肢体缺血模型中的侧支增强过程。左股动脉切除后，左臀下动脉与左腘动脉之间形成侧支血管主要路线，位于左尾骨股肌内。根据研究结果，我们在股动脉切除后28天选择性地将碱性成纤维细胞生长因子（bFGF）或骨髓单核细胞（MN）注射到左侧尾骨股肌或左侧内收肌。给予bFGF或MN后28天，与内收肌相比，尾骨股肌分娩后肢体缺血明显改善，表明分娩靶点的选择是促进有效侧支增强的重要因素。

项目成果

A Calcium-Cross-Linked Hydrogel Based on Alginate-Modified Atelocollagen Functions as a Scaffold Material

• DOI: 10.1163/092050611x555678
• 发表时间: 2012-01
• 期刊: Journal of Biomaterials Science, Polymer Edition
• 作者: W. Kamimura;Rieko Hattori;H. Koyama;T. Miyata;T. Takato

Impact of polyplex micelles installed with cyclic RGD peptide as ligand on gene delivery to vascular lesions

• DOI: 10.1038/gt.2011.74
• 发表时间: 2012-01-01
• 期刊: GENE THERAPY
• 影响因子: 5.1
• 作者: Kagaya, H.;Oba, M.;Miyata, T.
• 通讯作者: Miyata, T.

Inverse association between the existence of coronary artery disease and progression of abdominal aortic aneurysm

冠状动脉疾病的存在与腹主动脉瘤进展之间呈负相关

• DOI: 10.1016/j.atherosclerosis.2012.02.031
• 发表时间: 2012
• 期刊: Atherosclerosis
• 影响因子: 5.3
• 作者: Nakayama A;Miyata T
• 通讯作者: Miyata T

自家単核球細胞を用いた血管新生療法におけるデリバリー・ターゲットの重要性

使用自体单核细胞进行血管生成治疗时递送靶标的重要性

• 发表时间: 2013
• 作者: 根本卓;宮田哲郎ら
• 通讯作者: 宮田哲郎ら

Selective and Sustained Delivery of bFGF for the Development of Collatoral Vessels

选择性和持续输送 bFGF 以促进抵押血管的发育

• 发表时间: 2010
• 作者: 橋本亮太;武田雅俊;Koyama H
• 通讯作者: Koyama H