Long term culture and regulation of differentiation of germ cells from the testis in domestic species

家养物种睾丸生殖细胞的长期培养和分化调节

• 批准号: 22380150
• 负责人: IMAI Hiroshi
• 金额: $ 12.65万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380150/
• 关键词: 発生工学; 生殖幹細胞

A long-term culture of male primitive germ cells from bovine testis has been examined. Bovine primitive germ cells can proliferate and survive in culture for over 2 months in the condition with serum-free components and on poly-L-lysine-coated dish. The resulting cell colonies have both pluripotent and stem cell potent characteristics. After culture of cells in the presence of growth factors, cells are differentiated into spermatogonia.

已经检查了牛睾丸的雄性原始生殖细胞的长期培养。牛原始生殖细胞可以通过无血清成分和涂在多酰胺涂层的菜肴中在培养中增殖和存活2个月以上。所得的细胞菌落具有多能和干细胞有效特征。在存在生长因子的情况下细胞培养后，细胞被分化为精子症。

项目成果

Identification of reprogramming factors associated with a pluripotent potential of porcine and bovine spermatic stem cells in culture

鉴定与培养的猪和牛精索干细胞的多能潜能相关的重编程因子

• 发表时间: 2012
• 期刊: The Proceeding of The 16th Word Congress on In Vitro Fertilization
• 作者: Kim. SM;Fujihara. M;Goel. S;Sahare. M;Minami. N;Yamada. M;Imai. H
• 通讯作者: Imai. H

Determination of distinct germ cell commitment by the specific affinity of the lectin DBA during early development of gonocytes in neonatal mice testis

新生小鼠睾丸性母细胞早期发育过程中凝集素 DBA 的特异性亲和力测定不同生殖细胞的定向

• 发表时间: 2012
• 作者: KimSM;Fujihara M;Goel S;Minami N;Yamada M and Imai H
• 通讯作者: Yamada M and Imai H

Characterization and in vitro culture of male germ cells from developing bovine testes

发育中牛睾丸雄性生殖细胞的表征和体外培养

• 发表时间: 2011
• 期刊: Journal of Reproduction and Development
• 影响因子: 1.8
• 作者: Fujihara M;Kim SM;Minami N;Yamada M and Imai H
• 通讯作者: Yamada M and Imai H

Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a

蛋白激酶 A 通过 G9a 的表观遗传调控决定早期分化的时间

• 期刊: Cell Stem Cell
• 影响因子: 23.9
• 作者: Yamamizu K;Fujihara M;Tachibana M;Katayama S;Takahashi A;Hara E;Imai H;Shinkai Y;Yamashita JK
• 通讯作者: Yamashita JK

家畜ならびに大型実験動物における多能性幹細胞株の樹立と応用

家畜及大型实验动物多能干细胞系的建立及应用

• 发表时间: 2010
• 作者: Kozue Uchio-Yamada;Kyoko Sawada;Kotaro Tamura;Sumie Katayama;Youko Monobe;Yoshie Yamamoto;Atsuo Ogura;Noboru Manabe;築山智之・今井 裕
• 通讯作者: 築山智之・今井 裕