Studies for molecular mechanism of meiotic-competence-acquisition in porcine growing oocytes

猪生长卵母细胞减数分裂能力获得的分子机制研究

• 批准号: 22380147
• 负责人: NAITO Kunihiko
• 金额: $ 12.65万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380147/
• 关键词: 応用動物; 遺伝子; 畜産学; 成長途上卵; 発生能; 哺乳類卵; cAM依存性キナーゼ(PKA); AKAP; cAMP依存性キナーゼ(PKA); Wee1B

Mammalian growing oocytes cannot resume CDC2-activity-mediated meiotic maturation, even if they are grown up to 90% of their final size. In the present study, the molecular mechanisms of this incompetence were analyzed using porcine oocytes.As the results, it was revealed that porcine growing oocytes was inhibited their meiotic maturation by the two mechanisms, one was the insufficient CDC2 amount and the another one was the suppression of CDC2 activation by the regulation of PKA localization. In addition, the differences of PKA regulatory subunits (PKA-Rs) and A kinase anchoring proteins (AKAPs), which were involved in the control of PKA localization, were suggested between fully-grown and growing oocytes. I provided new insight that the change of PKA regulation mechanism during oocyte growth phase was the principle for the acquisition of meiotic competence in mammalian growing oocytes.

哺乳动物生长的卵母细胞无法恢复Cdc2活性介导的减数分裂成熟，即使它们长大了最终大小的90％。在本研究中，使用猪卵母细胞分析了这种无能的分子机制。结果，结果表明，猪的生长卵母细胞被两种机制抑制了它们的减数分裂成熟，一种是CDC2数量不足，而另一种是对Cdc2的抑制受到CDC2的抑制，而不是PKA的抑制。此外，建议在完全生长的卵母细胞和生长的卵母细胞之间，建议参与PKA锚定蛋白（AKAP）的PKA调节亚基（PKA-RS）和激酶锚定蛋白（AKAP）的差异。我提供了新的见解，即卵母细胞生长阶段PKA调节机制的变化是在哺乳动物生长卵母细胞中获得减数分裂能力的原理。

项目成果

Cdk7 and Cyclin H, but not Mat1, are involved in meiotic resumption in porcine immature oocyte.

Cdk7 和 Cyclin H（但不包括 Mat1）参与猪未成熟卵母细胞减数分裂的恢复。

• 发表时间: 2011
• 作者: Kozue Uchio-Yamada;Kyoko Sawada;Kotaro Tamura;Sumie Katayama;Youko Monobe;Yoshie Yamamoto;Atsuo Ogura;Noboru Manabe;築山智之・今井 裕;Fujii W
• 通讯作者: Fujii W

The regulation mechanism of phosphorylation of CDC2 threonine 161 during porcine oocyte maturation

猪卵母细胞成熟过程中CDC2苏氨酸161磷酸化的调控机制

• 发表时间: 2012
• 作者: Fujii W;Nishimura T;Kano K;Koji Sugiura;K Naito K
• 通讯作者: K Naito K

ブタ成長途上卵における減数分裂能欠損の分子機構

猪蛋发育过程中减数分裂缺陷的分子机制

• 发表时间: 2010
• 作者: Fujihara M;Kim SM;Minami N;Yamada M;Imai H.;西村鷹則・藤井渉・加納聖・内藤邦彦
• 通讯作者: 西村鷹則・藤井渉・加納聖・内藤邦彦

Preimplantation-embryo-specific Cell-cycle Regulation is Attributable to a Low Expression of Retinoblastoma Protein Rather Than Its Phosphorylation

植入前胚胎特异性细胞周期调节归因于视网膜母细胞瘤蛋白的低表达而不是其磷酸化

• DOI: 10.1262/jrd.10-170o
• 发表时间: 2011
• 期刊: Journal of Reproduction and Development
• 影响因子: 1.8
• 作者: Egashira A;Kano K;Naito K
• 通讯作者: Naito K

ブタ卵成熟におけるMASTLの機能解析

MASTL在猪卵母细胞成熟中的功能分析

• 发表时间: 2013
• 作者: Shohei Aoki;et al.;西村鷹則
• 通讯作者: 西村鷹則