Genomics of a diamondback moth and contribution to clarify the insecticide resistance mechanism

小菜蛾的基因组学及其对阐明杀虫剂抗性机制的贡献

• 批准号: 22380041
• 负责人: YAMAMOTO Kimiko
• 金额: $ 12.15万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380041/
• 关键词: 昆虫分子生物学; コナガゲノム; Bt菌毒素抵抗性; コナガ; cDNA; ゲノム; Bt抵抗性

EST analysis and RNAseq were performed and more than 30,000 gene candidates were acquired. KONAGAbase that provides diamondback moth comprehensive transcriptomic and draft genomic sequences with useful annotation information was developed and opened to the public. The candidates of Bt toxin resistance related genes were found with genetic analysis. The result of gene expression profiling showed that the genes associated with medicine metabolism had the low relevance with Bt toxin resistance.

进行了EST分析和RNASEQ，并获得了30,000多个候选基因。开发并向公众开发了有用的注释信息，可提供有用的注释信息，可提供响尾蛇飞蛾全面的转录组和基因组序列草案。通过遗传分析发现了BT毒素抗性相关基因的候选。基因表达分析的结果表明，与医学代谢相关的基因与BT毒素耐药性的相关性很低。

项目成果

Genome analysis of agricultural pests 2 : Development of a genomic database of the diamondback moth and its application to genome analysis

农业害虫基因组分析2：小菜蛾基因组数据库的建立及其在基因组分析中的应用

• 发表时间: 2011
• 作者: Jouraku A;Yamamoto K;Kuwazaki S;Urio M;Suetsugu Y;Narukawa J;Miyamoto K;Kurita K;Kanamori H;Katayose Y;Matsumoto T;Noda H
• 通讯作者: Noda H

The development of the genomic database of the diamondback moth (Plutella xylostella) and genomic analysis using it.

小菜蛾（Plutella xylostella）基因组数据库的开发以及利用该数据库进行基因组分析。

• 作者: Kitahara R.;Ikeda Y.;Shimura H.;Masuta C.;and Ezawa T.;伊藤彰紀・大出高弘・後藤久美子・松田武・柳沼利信・新美輝幸;上樂明也
• 通讯作者: 上樂明也

殺虫剤抵抗性問題におけるゲノム情報基盤の活用

利用基因组信息基础设施解决杀虫剂抗性问题

• 作者: Vu;V.B.;Takino;M.;Murata;T.;Nakayashiki;H.;上樂明也
• 通讯作者: 上樂明也

Genome analysis of agricultural pests 2:Development of a genomic database of the diamondback moth and its application to genome analysis

农业害虫基因组分析2：小菜蛾基因组数据库的建立及其在基因组分析中的应用

• 发表时间: 2011
• 作者: Jouraku A;Yamamoto K;Kuwazaki S;Urio M;Suetsugu Y;Narukawa J;Miyamoto K;Kurita K;Kanamori H;Katayose Y;Matsumoto T,Noda H
• 通讯作者: Matsumoto T,Noda H

Current status of the development of the diamondback moth database

小菜蛾数据库的发展现状

• 发表时间: 2011
• 作者: Jouraku A;Yamamoto K;Kuwazaki S;Urio M;Suetsugu Y;Narukawa J;Miyamoto K;Kurita K;Kanamori H;Katayose Y;Matsumoto T;Noda H
• 通讯作者: Noda H