Construction of disease-resistant plants by controlling the suppressor function of avirulence effector

控制无毒效应子的抑制功能构建抗病植物

• 批准号: 22380029
• 负责人: TSUYUMU Shinji
• 金额: $ 11.9万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380029/
• 关键词: カンキツかいよう病菌; PthAエフェクター; Pectin methyl esterase 前駆体; RIN4; UPAボックス; 非病原力遺伝子; AvrBs3ファミリー; PthA; PME前駆体; サプレッサー; 耐病性植物; PthA因子; Pectin methyl esterase前駆体; 組換え植物; 植物病害耐性; バイオテクノロジー; 細菌; 遺伝子; かいよう形成因子; カンキツ類

We had found that avirulence factors possess the suppressor function in addition to the common eliciting function of cultivar-specific resistance responses. PthA, a canker-forming effector with homology to the avirulence effector (AvrBs3) group commonly found in xanthomonads, was found to possess the suppressor function too. Here we found that PthA can bind specifically to the precursor for pectin methyl esterase (PME) of Citrus led to the new strategy to create the canker-resistant Citrus by the introduction of the PME precursors of other plant sources together with the introduction of mutation into the citrus PME precursor or gene silencing of PME precursor gene of Citrus by RNA interference. We suggest this modification of the suppressor activities of avirulence effectors by modification of their binding protein in the host plants as a new strategy for the construction of disease-resistant plants.

我们发现，无毒因子除了具有品种特异性抗性反应的常见引发功能外，还具有抑制功能。 PthA 是一种与黄单胞菌中常见的无毒效应子 (AvrBs3) 组同源的溃疡形成效应子，也被发现具有抑制功能。在这里，我们发现PthA可以与柑橘的果胶甲酯酶（PME）前体特异性结合，从而产生了通过引入其他植物来源的PME前体以及引入突变来创造抗溃疡病柑橘的新策略。柑橘PME前体或通过RNA干扰对柑橘PME前体基因进行基因沉默。我们建议通过修饰宿主植物中无毒效应子的结合蛋白来修饰无毒效应子的抑制活性，作为构建抗病植物的新策略。

项目成果

KdgR, an ICIR family transcriptional regulator, inhibits virulence mainly by repression of hrp genes in Xanthomonas oryzae pv.oryzae

KdgR 是 ICIR 家族转录调节因子，主要通过抑制米黄单胞菌 (Xanthomonas oryzae pv.oryzae) 中的 hrp 基因来抑制毒力

• 发表时间: 2011
• 期刊: J.Bacteriol.
• 作者: Lu;Y.;Islam;M.R.;Hirata;H.Tsujoimu;S.
• 通讯作者: S.

Induction of defense mechanisms on harvested mangoes by UV-C irradiation

UV-C 照射诱导收获芒果的防御机制

• 发表时间: 2013
• 期刊: Acta Horticult.
• 作者: Srepon;K.;S. Tsuyumu
• 通讯作者: S. Tsuyumu

Genome Sequence of the Plant-pathogenic bacterium Dickeya dadantii

植物病原菌Dickeya dadanii的基因组序列

• 发表时间: 2011
• 期刊: J. Bacteriol
• 作者: Jeremy D. Glasner;C-H. Yang;S. Tsuyumu;and Nicole T. Perna
• 通讯作者: and Nicole T. Perna

Role of PhoP-PhoQ two-component system in pellicle formation, virulence and survival in harsh environments of Dickeya dadantii 3937

PhoP-PhoQ 双组分系统在 Dickeya dadantii 3937 恶劣环境中的菌膜形成、毒力和生存中的作用

• 发表时间: 2012
• 期刊: J.Gen.Plant Pathol.
• 作者: Haque;M.M.;Hirata;H.;Tsuyumu;S.
• 通讯作者: S.

Mollecular Biological aand Nano-tecchnological sstudies on postharveest diseases

采后病害的分子生物学和纳米技术研究

• 发表时间: 2013
• 期刊: Acta Horticuul
• 作者: Tsuyumu;SS. and S. Tssuyumu
• 通讯作者: SS. and S. Tssuyumu