Design and creation of condition-responsive nano-drug-carriers involving by the step-wise construction method using intelligent nucleic acids

利用智能核酸的逐步构建方法设计和创建条件响应性纳米药物载体

• 批准号: 21710221
• 负责人: KUWAHARA Masayasu
• 金额: $ 3万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21710221/
• 关键词: 核酸; 有機化学; バイオテクノロジー; 生体生命情報学

Intelligent nucleic acids that can release/capture particular drug depending on the condition were created by designing and modifying drug-binding aptamers. Moreover, modified nucleotides were enzymatically incorporated to them by the post-modification method. The incorporation efficiencies were varied significantly depending on sequences around 3'-end of oligonucleotides tethered and chemical structures of modified nucleotides incorporated, which could be improved to some extent, by use of genetically modified polymerases.

通过设计和修改药物结合适体创建的智能核酸可以根据条件释放/捕获特定药物。此外，修饰的核苷酸是通过变化后酶掺入的。融合效率的变化很大，具体取决于寡核苷酸的3末端序列固定的寡核苷酸束缚和化学核苷酸的化学结构，可以通过使用遗传修饰的聚合酶在某种程度上改进。

项目成果

Molecular evolution of functional nucleic acids with chemical modifications.

• DOI: 10.3390/molecules15085423
• 发表时间: 2010-08-09
• 期刊: Molecules (Basel, Switzerland)
• 作者: Kuwahara M;Sugimoto N
• 通讯作者: Sugimoto N

Synthesis of acridonyl nucleoside analog and its fluorescent properties and substrate properties on polymerase reaction.

吖啶酮核苷类似物的合成及其荧光性质和聚合酶反应底物性质。

• 发表时间: 2010
• 作者: Masayasu Kuwahara;Yuya Kasahara;Keisuke Anahara;Yuta Irisawa;Masayoshi Tajima;Hiroaki Ozaki;Hiroaki Sawai;Akio Sugiyama.
• 通讯作者: Akio Sugiyama.

架橋型ヌクレオチドの3'末キャッピングによるDNAアプタマーのヒト血清中に おける安定性の評価

通过交联核苷酸 3 封端评估 DNA 适体在人血清中的稳定性

• 发表时间: 2010
• 作者: 笠原勇矢;北爪峻輔;桑原正靖;尾崎広明;澤井宏明;森廣邦彦;小比賀聡;今西武
• 通讯作者: 今西武

Enzymatic synthesis of artificial nucleic acids by various mutants of KOD DNA polymerase

KOD DNA聚合酶的各种突变体酶促合成人工核酸

• 发表时间: 2010
• 作者: Kuwahara M.;Takano Y.;Kasahara Y;Sugiyama A.;Ozaki H.;Sawai H
• 通讯作者: Sawai H

Syntheses and properties of acridonelabeled oligonucleotide analogs with two different backbone structures.

具有两种不同主链结构的吖啶酮标记寡核苷酸类似物的合成和性质。

• 发表时间: 2010
• 作者: Yuya Kasahara;Yuta Irisawa;Hidetaka Okonogi;Masayoshi Tajima;Atsushi Kobayashi;Masayasu Kuwahara;Hiroaki Ozaki;Seiji Tobita.
• 通讯作者: Seiji Tobita.