Development of durable gene expression systems comprising apatitecements or -particles including DNA complex

开发包含磷灰石水泥或颗粒（包括 DNA 复合物）的持久基因表达系统

• 批准号: 21700494
• 负责人: ITO Tomoko
• 金额: $ 2.75万
• 依托单位: Musashino University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700494/
• 关键词: ナノバイオ材料; 遺伝子治療; 放出制御; ハイドロキシアパタイト

The apatite-cements and -nanocapsule including DNA complex particles were degraded by osteoclast, and the DNA complex was continuously released. When they were injected peri-tumorally into tumor-bearing mice, significant tumor-growth suppression effect was observed, and in some cases, complete disappearance of tumor was achieved. They would be expected as a novel sustained-gene expression device.

含有DNA复合物颗粒的磷灰石水泥和纳米胶囊被破骨细胞降解，并且DNA复合物不断释放。当将它们注射到荷瘤小鼠的瘤周时，观察到显着的肿瘤生长抑制作用，在某些情况下，肿瘤甚至完全消失。它们有望成为一种新型的持续基因表达装置。

项目成果

Analysis of the surface structure of DNA/polycation/hyaluronic acid ternary complex by Raman microscpy

拉曼显微镜分析DNA/聚阳离子/透明质酸三元复合物的表面结构

• 发表时间: 2010
• 期刊: Jouenal of Pharmaceutial and Biomedical Analysis 51
• 作者: Kazufumi Nakamura;Daiji Miura;Ken Hashimoto;Hiroko Toyota;Hiroshi Okuyama;Juichiro Shimizu;Wakako Sumida;Satoshi Nagase;Kunihisa Kohno;Hiroshi Morita;Kengo Kusano;Naoto Yagi;Hiroshi Ito;Tomoko Ito
• 通讯作者: Tomoko Ito

Injectable self-setting apatite cement for DNA/PEI/hyaluronic acid ternary complex-releasing system and therapeutic effect in tumor model mice

DNA/PEI/透明质酸三元复合物释放系统注射用自凝磷灰石骨水泥及其对肿瘤模型小鼠的治疗作用

• 发表时间: 2011
• 作者: Y.Yokoyama;M.Umezaki;T.Kishioka;E.Tamiya;Y.Takamura;Tomoko Ito
• 通讯作者: Tomoko Ito

DNA complex-releasing system by apatite cement for gene therapy Universita' del Piemonte Orientale "AmedeoAvogadro"

用于基因治疗的磷灰石水泥 DNA 复合物释放系统 Universita del Piemonte Orientale "AmedeoAvogadro"

• 发表时间: 2011
• 作者: 向後雄太;小幡亜希子;春日敏宏;橋本謙;Tomoko Ito
• 通讯作者: Tomoko Ito

DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice

• DOI: 10.1016/j.biomaterials.2009.12.032
• 发表时间: 2010-04-01
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Ito, Tomoko;Yoshihara, Chieko;Koyama, Yoshiyuki
• 通讯作者: Koyama, Yoshiyuki

pDNA/PEI/コンドロイチン硫酸 凍結乾燥製剤の動物臨床への応用第10回遺伝子・デリバリー研究会シンポジウム

pDNA/PEI/硫酸软骨素冻干制剂在动物临床实践中的应用第十届基因与传递研究组研讨会

• 发表时间: 2010
• 作者: Sungho Lee;Akiko Obata;Toshihior Kasuga;芳原智恵子
• 通讯作者: 芳原智恵子