Study on the mechanism of the differentiation control of osteoblast and osteoclast based on the surface characteristics of biomaterials for hard tissues
基于硬组织生物材料表面特性的成骨细胞和破骨细胞分化控制机制研究
基本信息
- 批准号:21700485
- 负责人:
- 金额:$ 2.83万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Young Scientists (B)
- 财政年份:2009
- 资助国家:日本
- 起止时间:2009 至 2010
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The purpose of this study was to study the surface characteristics of polarized hydroxyapatite (HA) and the effects of electrical stimulation by polarization on osteoblast behavior in vitro in order to investigate the mechanism of the osteoconductive enhancement. Based on the material analysis, the surface characteristics revealed that electrical polarization had no effect on surface roughness, crystallinity, and constituent elements. According to contact angle measurement, electrically polarized HA, which provides two kinds of surfaces, negatively charged HA (N-HA) and positively charged HA (P-HA), was even more hydrophilic than that of normal HA. Morphological observations and quantitative analyses revealed that the typical adhered cells had a round shape on the HA but had a spindle or fan-like spreading configuration on the N-HA and the P-HA. The cell areas positively stained for actin, which indicates the degree of cell spreading, were distinctly larger on the N-HA and P-HA than that on the HA. In conclusion, electrical polarization improved the wettability of the HA surface and affected osteoblastic adhesion, especially spreading.
这项研究的目的是研究偏振羟基磷灰石(HA)的表面特征,以及极化对体外成骨细胞行为的电刺激的影响,以研究骨反导增强的机制。基于材料分析,表面特征表明,电化极化对表面粗糙度,结晶度和成分元件没有影响。根据接触角度测量,具有两种表面,带负电荷的HA(N-HA)和带正电的HA(P-HA)的电偏光HA比正常HA的亲水性更高。形态学观察结果和定量分析表明,典型的粘附细胞在HA上具有圆形,但在N-HA和P-HA上具有纺锤体或风扇状的扩散构型。在N-HA和P-HA上,肌动蛋白阳性染色的细胞区域明显大于HA上的细胞区域。总之,电化极化改善了HA表面的润湿性,并影响了成骨细胞粘附,尤其是扩散。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Efficacy of polarized hydroxyapatite and silk fibroin composite dressing on epidermal recovery from full-thickness porcine skin wounds
极化羟基磷灰石与丝素蛋白复合敷料对猪全层皮肤创面表皮恢复的影响
- DOI:
- 发表时间:2009
- 期刊:
- 影响因子:0
- 作者:R.Okabayashi;M.Nakamura;T.Okabayashi;Y.Tanaka;A.Nagai;K.Yamashita
- 通讯作者:K.Yamashita
セラミックバイオマテリアルの科学("セラミックスバイオマテリアル")(岡崎正之、山下仁大編)第3章
陶瓷生物材料科学(《陶瓷生物材料》)(冈崎雅之、山下仁主编)第3章
- DOI:
- 发表时间:2009
- 期刊:
- 影响因子:0
- 作者:C.Kojima;S.Tsumura;A.Harada;K.Kono;中村美穂
- 通讯作者:中村美穂
Osteoblast Adhesion and Differentiation Culltured on Polarized Hydroxyapatite
极化羟基磷灰石上培养的成骨细胞粘附和分化
- DOI:
- 发表时间:2009
- 期刊:
- 影响因子:0
- 作者:中村美穂;et al
- 通讯作者:et al
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NAKAMURA Miho其他文献
NAKAMURA Miho的其他文献
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{{ truncateString('NAKAMURA Miho', 18)}}的其他基金
Why human aging process varies - Exploring the diverse aging processes of our evolutionary cousin, the chimpanzees
为什么人类衰老过程各不相同 - 探索我们的进化表亲黑猩猩的多样化衰老过程
- 批准号:
16KT0006 - 财政年份:2016
- 资助金额:
$ 2.83万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of scaffolds to support mesenchymal stem cells for tissue engineering
开发支持组织工程间充质干细胞的支架
- 批准号:
15K20472 - 财政年份:2015
- 资助金额:
$ 2.83万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Looking at Human longevity in comparison with that of chimpanzees
与黑猩猩的寿命相比,人类的寿命
- 批准号:
25540056 - 财政年份:2013
- 资助金额:
$ 2.83万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
International analysis on corporate internal information disclosure in financial performance reporting
企业财务业绩报告内部信息披露的国际分析
- 批准号:
22730357 - 财政年份:2010
- 资助金额:
$ 2.83万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Facial recognition in patients with Williams syndrome-an MEG study-
威廉姆斯综合征患者的面部识别——一项 MEG 研究——
- 批准号:
15591152 - 财政年份:2003
- 资助金额:
$ 2.83万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Development of scaffold to promote mesenchymal stem cells for tissue regeneration(Fostering Joint International Research)
开发促进间充质干细胞组织再生的支架(促进国际联合研究)
- 批准号:
15KK0299 - 财政年份:2016
- 资助金额:
$ 2.83万 - 项目类别:
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
Regulation of bone-associated cells differentiation and function by micro configuration of material surface
通过材料表面的微结构调节骨相关细胞的分化和功能
- 批准号:
26462263 - 财政年份:2014
- 资助金额:
$ 2.83万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Enhancement of the cell activities on the carbonate apatite bone substitute by the conditioning of the sintering temperature
通过调节烧结温度增强碳酸盐磷灰石骨替代物的细胞活性
- 批准号:
25463060 - 财政年份:2013
- 资助金额:
$ 2.83万 - 项目类别:
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Histological examination of bone regeneration induced by mesenchymal stem cells: analyses of cell kinetics and bone quality
间充质干细胞诱导骨再生的组织学检查:细胞动力学和骨质量分析
- 批准号:
24792188 - 财政年份:2012
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$ 2.83万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Histological examination of bone regeneration induced by biomaterials mingled with mesenchymal stem cells
生物材料与间充质干细胞混合诱导骨再生的组织学检查
- 批准号:
22791962 - 财政年份:2010
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$ 2.83万 - 项目类别:
Grant-in-Aid for Young Scientists (B)