Study on the mechanism of the differentiation control of osteoblast and osteoclast based on the surface characteristics of biomaterials for hard tissues

基于硬组织生物材料表面特性的成骨细胞和破骨细胞分化控制机制研究

• 批准号: 21700485
• 负责人: NAKAMURA Miho
• 金额: $ 2.83万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700485/
• 关键词: バイオマテリアル; 骨芽細胞; 破骨細胞; 表面科学特性; ぬれ性; バイオセラミックス; 細胞接着; 細胞遊走; 細胞分化; 表面解析; 細胞増殖

The purpose of this study was to study the surface characteristics of polarized hydroxyapatite (HA) and the effects of electrical stimulation by polarization on osteoblast behavior in vitro in order to investigate the mechanism of the osteoconductive enhancement. Based on the material analysis, the surface characteristics revealed that electrical polarization had no effect on surface roughness, crystallinity, and constituent elements. According to contact angle measurement, electrically polarized HA, which provides two kinds of surfaces, negatively charged HA (N-HA) and positively charged HA (P-HA), was even more hydrophilic than that of normal HA. Morphological observations and quantitative analyses revealed that the typical adhered cells had a round shape on the HA but had a spindle or fan-like spreading configuration on the N-HA and the P-HA. The cell areas positively stained for actin, which indicates the degree of cell spreading, were distinctly larger on the N-HA and P-HA than that on the HA. In conclusion, electrical polarization improved the wettability of the HA surface and affected osteoblastic adhesion, especially spreading.

本研究的目的是研究极化羟基磷灰石（HA）的表面特性以及极化电刺激对体外成骨细胞行为的影响，以探讨其骨传导增强的机制。根据材料分析，表面特性表明电极化对表面粗糙度、结晶度和组成元素没有影响。根据接触角测量，电极化HA提供两种表面，带负电的HA（N-HA）和带正电的HA（P-HA），比普通HA更亲水。形态学观察和定量分析表明，典型的贴壁细胞在HA上呈圆形，但在N-HA和P-HA上呈纺锤形或扇形铺展形态。肌动蛋白呈阳性染色的细胞面积（表明细胞铺展的程度）在 N-HA 和 P-HA 上明显大于在 HA 上。总之，电极化改善了HA表面的润湿性并影响成骨细胞的粘附，尤其是铺展。

项目成果

Efficacy of polarized hydroxyapatite and silk fibroin composite dressing on epidermal recovery from full-thickness porcine skin wounds

极化羟基磷灰石与丝素蛋白复合敷料对猪全层皮肤创面表皮恢复的影响

• 发表时间: 2009
• 期刊: Journal of Biomedical Materials Research Part B-Applied Biomaterials 90B[2]
• 作者: R.Okabayashi;M.Nakamura;T.Okabayashi;Y.Tanaka;A.Nagai;K.Yamashita
• 通讯作者: K.Yamashita

セラミックバイオマテリアルの科学("セラミックスバイオマテリアル")(岡崎正之、山下仁大編)第3章

陶瓷生物材料科学（《陶瓷生物材料》）（冈崎雅之、山下仁主编）第3章

• 发表时间: 2009
• 作者: C.Kojima;S.Tsumura;A.Harada;K.Kono;中村美穂
• 通讯作者: 中村美穂

Osteoblast Adhesion and Differentiation Culltured on Polarized Hydroxyapatite

极化羟基磷灰石上培养的成骨细胞粘附和分化

• 发表时间: 2009
• 作者: 中村美穂;et al
• 通讯作者: et al

分極水酸アパタイトを含有するフィプロイン複合材料に体する細胞応答性

含极化羟基磷灰石的菲蛋白复合材料的细胞响应性

• 发表时间: 2010
• 作者: 中村美穂;ら
• 通讯作者: ら

Polarization and microstructural effects of ceramic hydroxyapatite electrets

• DOI: 10.1063/1.3265429
• 发表时间: 2010-01
• 期刊: Journal of Applied Physics
• 影响因子: 3.2
• 作者: Yumi Tanaka;Takeshi Iwasaki;Miho Nakamura;A. Nagai;K. Katayama;K. Yamashita