Therapeutic application to neonatal PVL of amnion-derived cells

羊膜来源细胞在新生儿PVL中的治疗应用

• 批准号: 21791029
• 负责人: SASAKI Yoshihito
• 金额: $ 2.75万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791029/
• 关键词: 胎児医学; 羊膜細胞; SP細胞; リアルタイムPCR

We have obtained human amnion during caesarian section from 20 volunteers. After the culture, amniotic cells were sorted by flow cytometry. The cell fraction that we consider containing stem cells is no more than 0.02% of total cells. A neonatal rat model with hypoxic-ischemic brain injury was prepared. We injected these cells suspended in a solution into the lateral ventricle of the brain. We also applied the cells as a form of cell sheet directly onto the injured cerebral cortex. Neuroprotective effects of the cell therapies have not been observed so far. We continue analyzing other methods of cell delivery and other cell sources.

我们在20名志愿者的凯撒分区期间获得了人类羊膜。培养后，通过流式细胞仪对羊水细胞进行分选。我们考虑包含干细胞的细胞分数不超过总细胞的0.02％。制备了具有低氧 - 缺血性脑损伤的新生大鼠模型。我们将这些细胞注射到溶液中，进入大脑的外侧心室。我们还将细胞作为细胞片的形式直接应用于受伤的大脑皮层。到目前为止，尚未观察到细胞疗法的神经保护作用。我们继续分析其他细胞输送和其他细胞来源的方法。

项目成果

Intracranial Injection of Fetal Membranous Mesenchymal Stem Cells Following Hypoxic Ischemic Encephalopathy in Neonatal Rats.

新生大鼠缺氧缺血性脑病后颅内注射胎儿膜间充质干细胞。

• 发表时间: 2008
• 作者: Keiko Ueda;Kazuhiko Harada;Jun Yoshimatu;Shinji Kasturagi;Shin Ishikane;Noritoshi Nagaya;Tomoaki Ikeda
• 通讯作者: Tomoaki Ikeda