Effect of mixed infection by periodontopathic bacteria-increase of abscess formation on mice and responses by hosts.

牙周病细菌混合感染的影响——脓肿形成增加对小鼠和宿主的反应。

• 批准号: 13672189
• 负责人: YONEDA Masahiro
• 金额: $ 1.92万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672189/
• 关键词: Porphyromonas gingivalis; Bacteroides forsythsus; mixed infection; murine abscess model; gingipain; T cell; cytokine; chemokine; システインプロテアーゼ

We examined the effect of mixed infection of Porphyromonas gingivalis and Bacteroides forsythus on abscess formation using murine abscess model. When these two bacteria were injected together, they exhibited synergistic effect comparing to the mono-infection by each bacterium. The mutant strains of P. Gingivalis devoid of gingipains exhibited reduced abscess forming abilities comparing to the wild strain. When these mutant strains were used instead of the wild strain in the mixed infection experiments, they only showed additive effects. These results indicate that periodontopathogens exhibit synergism in the periodontal environment and gingipains are playing an important role in the mixed infection.We also examined the effect of P. gingivalis on T cells. The culture supernatant of this bacterium was found to have CD4-cleaving activity. And it was revealed that gingipains were playing an important role in this activity.We also revealed that cytokines, chemokines, and receptors for these are playing an important role in the marginal and periapical periodontitis. They were found to be associated with the progression of periodontitis in Papillon-Leffevre Syndrome, too. We also reported the function of cytokines in the rat experimental periapical periodontitis.

我们使用小鼠脓肿模型研究了牙龈卟啉单胞菌和福赛斯拟杆菌混合感染对脓肿形成的影响。当这两种细菌一起注射时，与每种细菌单独感染相比，它们表现出协同效应。与野生菌株相比，不含牙龈蛋白酶的牙龈卟啉单胞菌突变菌株表现出降低的脓肿形成能力。当在混合感染实验中使用这些突变株代替野生株时，它们仅表现出相加效应。这些结果表明牙周病原菌在牙周环境中表现出协同作用，牙龈蛋白酶在混合感染中发挥着重要作用。我们还检测了牙龈卟啉单胞菌对T细胞的影响。发现该细菌的培养上清液具有CD4切割活性。结果表明，牙龈蛋白酶在这一活动中发挥着重要作用。我们还发现，细胞因子、趋化因子及其受体在边缘牙周炎和根尖周炎中发挥着重要作用。研究发现它们也与 Papillon-Leffevre 综合征的牙周炎进展有关。我们还报道了细胞因子在大鼠实验性根尖周炎中的功能。

项目成果

K.Maeda, H.Anan et al.: "Immunology and bone formation of experimentally induced periapical lesions in rats"Proc.of International Conference on Dentin-Pulp Complex 2001. 19-23 (2002)

K.Maeda，H.Anan 等：“大鼠实验诱导根尖周病变的免疫学和骨形成”Proc.of International Conference on Dentin-Pulp Complex 2001. 19-23 (2002)

H.Kabashima, M.Yoneda et al.: "The presence of cytokine (IL-8, IL-1α, IL-1β)-producing cells in inflamed gingival tissue from a patient manifesting Papillon-Lefevre Syndrome (PLS). Cytokine 18:"CYTOKINE. 18. 121-126 (2002)

H.Kabashima、M.Yoneda 等人：“表现 Papillon-Lefevre 综合征 (PLS) 的患者发炎牙龈组织中存在产生细胞因子（IL-8、IL-1α、IL-1β）的细胞。细胞因子 18 ：“细胞因子。18。121-126（2002）

Y.Kitamura, M.Yoneda et al.: "Gingipains in the culture supernatant of Porphyromonas gingivalis cleave CD4 and CD8 on human T cells"J.Periodontal Res.. (In press).

Y.Kitamura、M.Yoneda 等人：“牙龈卟啉单胞菌培养上清液中的牙龈蛋白酶可裂解人 T 细胞上的 CD4 和 CD8”J.Periodontal Res..（正在出版）。

H.Kabashima, M.Yoneda et al.: "The presence of chemokine (MCP-1, MIP-1α, MIP-1β, IP-10, RANTES)-positive cells and chemokine receptor (CCR5, CXCR3)-positive cells in human gingival tissues"CYTOKINE. 20. 70-77 (2002)

H.Kabashima、M.Yoneda 等人：“趋化因子（MCP-1、MIP-1α、MIP-1β、IP-10、RANTES）阳性细胞和趋化因子受体（CCR5、CXCR3）阳性细胞的存在人牙龈组织”CYTOKINE. 20. 70-77 (2002)

H.Kabashima, M.Yoneda et al.: "The presence of cytokine (IL-8, IL-1α, IL-1β)-producing cells in inflamed gingival tissue from a patient manifesting Papillon-Lefevre Syndrome (PLS).Cytokine 18:"CYTOKINE. 18. 121-126 (2002)

H.Kabashima、M.Yoneda 等人：“表现 Papillon-Lefevre 综合征 (PLS) 的患者发炎牙龈组织中存在产生细胞因子（IL-8、IL-1α、IL-1β）的细胞。细胞因子 18 ：“细胞因子。18。121-126（2002）