Development of new real-time TRAP measurement method with nano-FIA system and its application

纳米FIA系统TRAP实时测量新方法的开发及其应用

• 批准号: 21790037
• 负责人: WADA Mitsuhiro
• 金额: $ 2.66万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790037/
• 关键词: 分析化学; 臨床化学; TRAP; ナノFIA; マイクロダイアリシス法; 抗酸化

Real time evaluation method of in vivo antioxidative effect in rat blood after administration of ascorbic acid (ASA) was studied. The antioxidative effect in small amount of samples (500nL) was measured by nano-flow injection analysis method with chemiluminescence detection hyphenated with microdialysis method. Antioxidative effects of blood microdialysates obtained from rats administered 50mg/kg ASA through different routes (intravein : i.v., intraperitoneal : i.p. and per os : p.o.) were evaluated. The increased effects were kept up till 360 min after administration. The AUC0-360 (antioxidative effect %×min) were 16,260±1,739 (i.v.), 12,873±2,152 (i.p.) and 6,668±2,251 (p.o.), respectively. On the other hand, antioxidative effects in brain microdialysates were not observed by the dose (50mg/kg). Additionally the fact, which ASA was the source of antioxidative effect in microdialysate, was cleared by disappearing of the effect by treatment with ascorbic acid oxidase. Furthermore, the concentration of ASA in microdialysate determined was correlated with that of antioxidative effect (r=0.915).

研究了给予抗坏血酸(ASA)后大鼠血液体内抗氧化作用的实时评价方法采用纳流注射分析法和化学发光检测联用微透析法测定了少量样品(500nL)的抗氧化作用。通过不同途径（静脉内：i.v.、腹膜内：腹腔注射和口服：给药后 360 分钟，AUC0-360（抗氧化作用%×分钟）分别为 16,260±1,739（静脉注射）、12,873±2,152（腹腔注射）和 6,668±。分别为 2,251（邮局）。剂量(50mg/kg)没有观察到脑微透析液中的抗氧化作用。此外，通过用抗坏血酸氧化酶处理，该作用消失，从而清除了ASA是微透析液中的抗氧化作用的来源的事实。测定的微透析液中 ASA 含量与抗氧化作用相关（r=0.915）。

项目成果

A Simple HPLC-fluorescence Method for Quantitation of Curcuminoids and Its Application to Turmeric Products

• DOI: 10.2116/analsci.25.385
• 发表时间: 2009-03-01
• 期刊: ANALYTICAL SCIENCES
• 影响因子: 1.6
• 作者: Zhang, Junwei;Jinnai, Sakie;Nakashima, Kenichiro
• 通讯作者: Nakashima, Kenichiro

健康食品素材の抗酸化能評価

保健食品原料的抗氧化能力评价

• 发表时间: 2009
• 作者: 和田光弘;ら
• 通讯作者: ら

Semi-micro flow injection analysis method for evaluation of quenching effect of health foods or food additive antioxidants on peroxynitrite

评价保健食品或食品添加剂抗氧化剂对过氧亚硝酸盐猝灭效果的半微流注射分析方法

• DOI: 10.1002/bio.1206
• 发表时间: 2011
• 期刊: Luminescence
• 影响因子: 2.9
• 作者: M.Wada;M.Kira;H.Kido;R.Ikeda;N.Kuroda;T.Nishigaki;K.Nakashima
• 通讯作者: K.Nakashima

マイクロダイアリシス-セミミクロフローインジェクション分析(MD-SMFIA)法によるアスコルビン酸投与後のラット血液抗酸化活性モニタリング

微透析-半微流注射分析（MD-SMFIA）法监测抗坏血酸给药后大鼠血液抗氧化活性

• 发表时间: 2010
• 作者: 中路洋輔;ら
• 通讯作者: ら

化学発光フローインジェクション分析法による西アジア原産植物の活性酸素種消去能評価

化学发光流动注射分析法评价西亚原产植物活性氧清除能力

• 发表时间: 2009
• 作者: 和田光弘;福永裕子;山形浩介;池田理恵;Suleiman M.Al-Khalil;中島憲一郎
• 通讯作者: 中島憲一郎