A Study on the Application to the Pulp Capping Agent of a DNA Film

DNA薄膜盖髓剂的应用研究

基本信息

批准号：
13672018
负责人：
YAMADA Kazuhiko
金额：
$ 2.56万
依托单位：
Fukuoka Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672018/
关键词：
DNA-Film Vital pulpotomy Pulp Capping Hydroxyapatite 覆髄 BMP アパタイトビーグル犬電顕的観察

项目摘要

Presently, vital pulpotomy using calcium hydroxide is widely performed in general clinical treatment, and dentin bridge formation is considered an indication of its success. However, since calcium hydroxide is a strong alkali, it has been reported to cause extensive necrosis in pulp tissue. Furthermore, the formation of new, hard tissue such as a dentin bridge is considered to indicate that toxic stimulation has been applied to the pulp. If pulp tissue could be preserved with less damage through the use of a biocompatible material, it would be considered to be extremely significant. Therefore, we investigated the use of artificial lipid-modified DNA, which has been reported to be moderately biodegradable, non-antigenic, and antibiotic, as such a material. Fukushima et al. showed that artificial lipid-modified DNA is a promising biodegradable biological material, and it is expected to be applied to dentistry. In this study, we evaluated its use as a pulp dressing material after vital pulpotomy. Since preliminary experiments revealed problems with the manipulability of DNA film itself, we applied uncoated and DNA-coated hydroxyapatite to pulpotomy surfaces of the bilateral upper and lower canine teeth in beagles and histopathologically examined pulp tissue changes. In the pulp treated with DNA-coated hydroxyapatite, inflammatory cell infiltration and calcification were milder than in those treated with uncoated hydroxyapatite. These findings suggest that DNA is not toxic to pulp tissue and even exhibits an anti-inflammatory activity. Moreover, as DNA can intercalate antibiotics and osteogenesis inducers, it may be applied in the future as a pulp dressing agent.

目前，使用氢氧化钙的活髓切断术在一般临床治疗中广泛进行，牙本质桥的形成被认为是其成功的标志。然而，由于氢氧化钙是强碱，据报道会导致牙髓组织广泛坏死。此外，新的硬组织（例如牙本质桥）的形成被认为表明牙髓已受到有毒刺激。如果通过使用生物相容性材料能够以较小的损伤保存牙髓组织，将被认为是极其有意义的。因此，我们研究了人工脂质修饰 DNA 作为此类材料的用途，据报道，这种材料具有适度的生物降解性、非抗原性和抗生素。福岛等人。表明人工脂质修饰DNA是一种很有前景的可生物降解生物材料，有望应用于牙科领域。在这项研究中，我们评估了其在活髓切断术后作为牙髓敷料材料的用途。由于初步实验揭示了DNA薄膜本身的可操作性问题，我们将未涂覆和DNA涂覆的羟基磷灰石应用于比格犬双侧上、下犬齿的活髓切断表面，并进行组织病理学检查牙髓组织的变化。在用DNA涂覆的羟基磷灰石处理的牙髓中，炎症细胞浸润和钙化比用未涂覆的羟基磷灰石处理的牙髓温和。这些发现表明 DNA 对牙髓组织没有毒性，甚至具有抗炎活性。此外，由于DNA可以嵌入抗生素和成骨诱导剂，因此未来可能会被用作牙髓敷料剂。