Linkage mapping and eQTL analysis of candidate resistant gene for pine wilt disease caused by wood nematode

木材线虫松材线虫病候选抗性基因连锁图谱及eQTL分析

• 批准号: 21780157
• 负责人: ISODA Keiya
• 金额: $ 2.91万
• 依托单位: Forestry and Forest Products Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780157/
• 关键词: 森林生産; 育種; 遺伝学; 林木育種; eQTL; 連鎖地図; 3'-RACE; マツノザイセンチュウ; 抵抗性; シングルジーン; リアルタイムPCR; 遺伝子発現; マツ; 3^'RACE; EST; SNP

3'-RACE was conducted using previously reported EST information of candidate resistant gene for pine wood nematode in order to determine 3'-end sequence of the ESTs. PCR primers were designed and single-genes were screened by sequencing haploid genome extracted from megagametophyto. Two primers were developed to amplify single-genes containing SNPs. Expression QTL analysis of pathogen related genes were conducted. EQTL of chitinase and PR-4 were detected in the same linkage group of phenotypic resistant trait, suggesting similar regulation factor was related to these genes and resistance

使用先前报道的松树线虫候选抗性基因的EST信息进行3'-Race，以确定EST的3'-End序列。设计了PCR引物，并通过从巨型植物学中提取的单倍体基因组来筛选单基因。开发了两个引物来扩增含有SNP的单一生成。对病原体相关基因的表达QTL分析。在同一表型抗性性状的相同连接组中检测到了几丁质酶和PR-4的EQTL，这表明相似的调节因子与这些基因有关，并且抗性有关