Effects or hypergravity on genes expressed in the rat inner car

超重力对大鼠内车基因表达的影响

基本信息

批准号：
13671778
负责人：
DOI Katsumi
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Recovery from balance disorders aster spaceflight is supposed.to reflect the readaptation to normal gravitational environment (1G). from microgravity, In the present study, to elucidate a role of otoconia in adaptation to altered gravity, we examined the effects of hypergravity (2G) on nor polo and synthesis of otoconia in young adult rats, Neither otoconial morphology nor otoconial synthesis was affected by up to 1 week exposure to hypergravity, These results suggest that changes in neurotransmission at the synapses or flit peripheral and/or contral vestibular system rather than the Changes in otoconial morphology and synthesis may be involved in adaptive mechanismsWe also assessed the changes in mRNA expression of glutamate receptors in the vestibular system of rats exposed to hypergravily for 2 h to 1 week using real-time quantitative RT-PCR, Tho expression of GluR2 and NRI in khe uvula/nodulus and NR1 in the medial vestibuiar nucleus increased after 2 h of hypergravity, The expresion of GluR2 in vestibular ganglion cells decreased after 1 week of hypergravity ; The results suggested that the animals adapted to the hypergravity by enhancing the crrebellar inhibition of the vestibular nucleus via an activation of the NR1 and GluR2 on tha Purkinje cells in uvula/nodules especially of the early phase following hypergravity, while in the later phase by reducing the neurotransmission between the vestibular hair cells and the primary vestibular neurons via down-regulation of the postsynaptic GluR2 in the vestibular periphery

从平衡失调中恢复紫苑太空飞行应该反映对正常重力环境（1G）的重新适应。在本研究中，为了阐明耳石在适应重力改变中的作用，我们研究了超重力（2G）对年轻成年大鼠耳石和耳石合成的影响，耳石形态和耳石合成均不受微重力影响。暴露于超重力长达 1 周，这些结果表明突触或外周和/或控制前庭系统的神经传递发生变化，而不是耳圆锥形态和合成可能参与适应性机制我们还使用实时定量RT-PCR、GluR2和NRI的Tho表达评估了暴露于超重力2小时至1周的大鼠前庭系统中谷氨酸受体mRNA表达的变化超重力2小时后，悬雍垂/结节中的KHE和前庭内侧核中的NR1增加，前庭中GluR2的表达超重力1周后神经节细胞减少；结果表明，动物适应超重力的方式是通过激活悬雍垂/结节中浦肯野细胞上的 NR1 和 GluR2 来增强前庭核的小脑抑制，特别是在超重力后的早期阶段，而在超重力的后期，通过减少通过下调前庭外周突触后 GluR2 来调节前庭毛细胞和初级前庭神经元之间的神经传递