Signaling pathway of Xenopus egg activation

非洲爪蟾卵激活的信号通路

• 批准号: 21770142
• 负责人: IWASAKI Tetsushi
• 金额: $ 2.91万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770142/
• 关键词: 細胞情報伝達機構; 発生・分化; シグナル伝達

We have previously demonstrated that Src tyrosine kinase plays an important role in the Xenopus egg activation. However, the molecular mechanism of egg activation is still unclear. Here, we investigated the activation mechanism and the physiological function of Src kinase in Xenopus egg fertilization. Biochemical and immunochemical analyses have revealed the involvement of heterotrimeric G proteins in sperm-induced Src activation. Moreover, we analyzed hnRNP K protein, which is known as a substrate of Src kinase in Xenopus egg fertilization. Our molecular biological and biochemical analyses showed that the hnRNP K binds to specific mRNAs in unfertilized egg, and that Src-mediated tyrosine phosphorylation acts as a trigger for their dissociation at egg activation.

我们以前已经证明，SRC酪氨酸激酶在爪蟾卵激活中起重要作用。然而，卵子激活的分子机制尚不清楚。在这里，我们研究了SRC激酶在异爪蟾卵受精中的激活机制和生理功能。生化和免疫化学分析揭示了异源三聚体G蛋白参与精子诱导的SRC激活。此外，我们分析了HNRNP K蛋白，该蛋白被称为Xenopus卵受精中SRC激酶的底物。我们的分子生物学和生化分析表明，HNRNP K与未施肥的卵中的特定mRNA结合，并且SRC介导的酪氨酸磷酸化是它们在卵激活时解离的触发因素。

项目成果

Physiology and gene expression of the rice landrace Horkuch under salt stress

• DOI: 10.1071/fp10198
• 发表时间: 2011-01-01
• 期刊: FUNCTIONAL PLANT BIOLOGY
• 影响因子: 3
• 作者: Lisa, Laisa A.;Elias, Sabrina M.;Seraj, Zeba I.
• 通讯作者: Seraj, Zeba I.

Evolutional and functional analysis of Xenopus STAT3

非洲爪蟾 STAT3 的进化和功能分析

• 发表时间: 2011
• 作者: Tetsushi Iwasaki;Sho Iguchi;Alexander Tokmakov;Yasuo Fukami.
• 通讯作者: Yasuo Fukami.

FM-AFM Study of Proteins on Lipid Rafts

脂筏上蛋白质的 FM-AFM 研究

• 发表时间: 2010
• 作者: Saeko Yanaka;Motonori Kudou;Yoshikazu Tanaka;Takumi Sasaki;Sumiyo Takemoto;Atsuko Sakata Yukio Hattori;Tomoyuki Koshi;Shiro Futaki;Kouhei Tsumoto;and Toshihiro Nakashima;Tomoki Sugihara
• 通讯作者: Tomoki Sugihara

Analysis of signal transduction in cell-free extracts and rafts of Xenopus eggs

爪蟾卵无细胞提取物和筏中的信号转导分析

• 发表时间: 2010
• 期刊: Methods 51(1)
• 作者: Tokmakov A.A.;Iwasaki T.;Sato KI.;Fukami Y.
• 通讯作者: Fukami Y.

Unfertilized Xenopus eggs die by apoptosis after MAPK inactivation

MAPK 失活后，未受精的非洲爪蟾卵因细胞凋亡而死亡

• 发表时间: 2009
• 作者: Tokmakov A;Alexander;Sho Iguchi;Tetsushi Iwasaki;Yasuo Fukami
• 通讯作者: Yasuo Fukami