Analysis of the antigen presentation machinery in malignant glioma cells

恶性胶质瘤细胞中抗原呈递机制的分析

• 批准号: 13671427
• 负责人: SATOH Eiji
• 金额: $ 0.9万
• 依托单位: University of Yamanashi (Faculty of Medicine)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671427/
• 关键词: malignant glioma; transporter associated with antigen processing; interferon-β; 悪性グリオーマ; transporter with associated with antigen processing

Transporter associated with antigen processing (TAP) is necessary for peptide transport and antigen presentation. We investigated a expression of TAP1 in malignant glioma cells and the effect of IFN-γ, IFN-β on the expression of TAP1. We also investigated the polymorphism of TAP1 promoter. We analyzed the sequence of TAP1 promoter in eight malignant glioma cells. Single nucleotide polymorphism occurred in six of eight malignant glioma cells. This polymorphism is a G-T substitution 446 bp upstream of the translation start of TAP1. The expression of TAP1 in all malignant glioma cells was very low. IFN-γ and IFN-β increased the protein and mRNA expression of TAP1. The polymorphism of TAP1 promoter did not have functional effects on the induction of TAP1 expression by IFN-γ and IFN-β.

抗原加工相关转运蛋白（TAP）是肽转运和抗原呈递所必需的，我们研究了恶性胶质瘤细胞中TAP1的表达以及IFN-γ、IFN-β对TAP1表达的影响。我们分析了八个恶性神经胶质瘤细胞中的TAP1启动子的序列，该多态性是G-T。 TAP1翻译起始点上游446 bp的取代在所有恶性胶质瘤细胞中的IFN-γ和IFN-β增加了TAP1的蛋白质和mRNA表达。TAP1启动子的多态性没有功能影响。 IFN-γ和IFN-β诱导TAP1表达的研究。