The control of bacterial infectious diseases by the hamony of immune system and nerve system

免疫系统与神经系统的协调控制细菌感染性疾病

• 批准号: 13670261
• 负责人: NAKANE Akio
• 金额: $ 2.37万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670261/
• 关键词: Listeria monocytogenes; Sympathetic nerve system; Epinephrine receptor; MIF; IFN-gamma; TNF-alpha; IL-10; Cortisol; 下垂体; 神経系; 細菌感染

The host defense mechanism against bacterial infections through catecholamine receptors was investigated. Furthermore, the role of macrophage migration inhibitory factor (MIF), which is known to mediate the crosstalk between immune system and nerve system, in bacterial infections was investigated.1. The regulatory mechanism by catecholamines in host defense against bacterial infections. The in vivo effects of antagonists and agonists to epinephrine receptors on host defense against Listeria monocytgenes infection and cytokine responses were investigated. When epinephrine receptor α antagonists were injected into mice, antilisterial resistance and productions of IFN-γ and TNF-α were suppressed. In contrast, epimephrine receptor β antagonists revealed no significant effect on antilisterial resistance and the cytokine production. These results suggest that antilisterial resistance and productions of IFN-γ and TNF-α may be regulated by epinephrine receptor α.2. The role of MIF in bacterial … More infections (1) Endogenous MIF levels in the infected organs were up-regulated by a lethal infection with L. monocytogenes. The administration of anti-MIF antibody into mice showed no significant effect on the fate of a sublethal infection with L. monocytogenes, whereas the antibody rescued the mice from a lethal infection with L. monocytogenes. (2) Endogenous IFN-γ and TNF-α production in the infected organs was up-regulated in the early phase but down-regulated in the late phase of lethal infection in anti-MIF antibody-treated mice. (3) Endogenous IL-10 production in the livers was up-regulated in anti-MIF antibody-treated mice. Abnormality of liver function was partially restored by the administration of anti-IL-10 antibody in anti-MIF antibody-treated mice. (4) The level of plasma cortisol was significantly low in anti-MIF antibody-treated mice, compared with that of the normal rabbit globulin-treated mice.These results suggest that MIF is involved in the pathogensis of a lethal infection with L. monocytogenes through the regulation of IL-10 production. Less

研究了宿主通过儿茶酚胺受体防御细菌感染的机制。此外，还研究了巨噬细胞迁移抑制因子（MIF）在细菌感染中介导免疫系统和神经系统之间的相互作用。1．研究了肾上腺素受体拮抗剂和激动剂对宿主抵抗单核细胞增多性李斯特菌感染和细胞因子反应的体内作用。将拮抗剂注射到小鼠体内，抗李斯特菌耐药性和 IFN-γ 和 TNF-α 的产生受到抑制，相反，表甲肾上腺素受体 β 拮抗剂对抗李斯特菌耐药性和细胞因子的产生没有显着影响。这些结果表明，抗李斯特菌耐药性和 IFN-α 的产生。 -γ 和 TNF-α 可能受肾上腺素受体 α 调节。2 MIF 在细菌感染中的作用 (1) 受感染器官中的内源性 MIF 水平为向小鼠注射抗 MIF 抗体对单核细胞增生李斯特菌亚致死感染的命运没有显着影响，而该抗体可以使小鼠免受单核细胞增生李斯特菌致命感染的影响。 (2)在抗MIF抗体处理的小鼠中，感染器官中内源性IFN-γ和TNF-α的产生在早期阶段上调，但在致死感染的后期阶段下调。 (3)在抗MIF抗体处理的小鼠中肝脏中内源性IL-10的产生上调通过在抗MIF抗体处理的小鼠中施用抗IL-10抗体部分地恢复了肝功能的异常。 (4)与正常兔球蛋白处理的小鼠相比，抗MIF抗体处理的小鼠血浆皮质醇水平显着降低。这些结果表明MIF参与了李斯特菌致死性感染的发病机制。单核细胞增多症通过调节 IL-10 的产生。

项目成果

Hiroshi Sashinami: "Effective induction of acquired resistance to Listeria monocytogenes by immunizing mice with in vivo-infected dendritic cells"Infection and Immunity. 71・1. 117-125 (2003)

Hiroshi Sashinami：“通过用体内感染的树突状细胞免疫小鼠来有效诱导对单核细胞增生李斯特氏菌的获得性抗性”感染和免疫71·1（2003）。

Suguru Hasegawa: "Dysregulation of interleukin-10 and interleukin-12 are involved in the reduced host resistance to Listeria monocytogenes infection in alymphoplastic aly mutant mice"FEMS Immunology and Medical Microbiology. 32・3. 111-117 (2002)

Suguru Hasekawa：“白细胞介素 10 和白细胞介素 12 的失调与淋巴细胞增生性 aly 突变小鼠的宿主对单核细胞增多性李斯特菌感染的抵抗力降低有关”FEMS 免疫学和医学微生物学 32·3 (2002)。

Hu D-I, Omoe K, Shimada Y, Nakane A, Shiangawa K.: "Induction of emetic response to staphylococcal enterotoxins in the house musk Shew (Surcus murinus)"Infection and Immunity. 71-2. 690-696 (2003)

Hu D-I、Omoe K、Shimada Y、Nakane A、Shiangawa K.：“在家麝香秀（Surcus murinus）中诱导对葡萄球菌肠毒素的催吐反应”感染和免疫。

Sanae Sasaki: "Role of gamma interferon and tumor necrosis factor-alpha in shiga toxin lethality"Microbial Pathogenesis. 33・1. 43-47 (2002)

佐佐木早苗：“γ干扰素和肿瘤坏死因子-α在志贺毒素致死性中的作用”微生物发病机制33・1（2002）。

Tomisato Miura: "Effect of 6-hydroxydopamine of host resistance against Listeria monocytogenes infection"Infection and Immunity. 69・12. 7234-7241 (2001)

三浦富里：“6-羟基多巴胺对单核细胞增多性李斯特菌感染的宿主抵抗力的影响”感染和免疫69・12（2001）。