Leneage analysis of postnatal neurogenesis in organotypic culture of hippocampus

海马器官型培养中出生后神经发生的谱系分析

• 批准号: 21500299
• 负责人: ISHIZUKA Toru
• 金额: $ 2.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500299/
• 关键词: 神経新生; 海馬; レトロウイルス; スライス培養; オプトジェネティクス; 細胞系譜; 神経科学; ニューロン

Using the retrovirus vectors encoding EGFP, we labeled proliferating cells in an organotypic slice culture of the postnatal rat hippocampus and tracked an EGFP-labeled single progenitor cell and its descendants as a lineage over a long period. We found that the newly generated neurons became frequently untraceable and this critical traceability period was 2-14 days. It is suggested that the newly generated neurons differentiate into mature dentate granule neurons once they have survived over the critical period. We also created novel genetic probes, which consist of an optogenetic or chemical actuator and an apoptosis detecting reporter. They would help us to reveal the processes that determine the fate of newly generated neurons in an activity-dependent manner.

使用编码 EGFP 的逆转录病毒载体，我们标记了出生后大鼠海马器官切片培养物中的增殖细胞，并长期跟踪了 EGFP 标记的单个祖细胞及其后代作为谱系。我们发现新生成的神经元经常变得无法追踪，这个关键的可追踪期是 2-14 天。这表明，新产生的神经元一旦存活过关键期，就会分化为成熟的齿状颗粒神经元。我们还创建了新型遗传探针，其由光遗传学或化学致动器和细胞凋亡检测报告基因组成。它们将帮助我们揭示以活动依赖性方式决定新生成神经元命运的过程。

项目成果

APACOP, a FRET apoptosis probe with manipulation of neuronal activity

APACOP，一种可操纵神经元活动的 FRET 凋亡探针

• 发表时间: 2011
• 作者: Daniel BL Teh;横瀬淳;王紅霞;酒井誠一郎;石塚徹;八尾寛
• 通讯作者: 八尾寛

Lineage analysis of postnatal neurogenesis in the slice culture of hippocampus

海马切片培养中出生后神经发生的谱系分析

• 发表时间: 2009
• 作者: Jun Yokose;Hiromu Yawo;Toru Ishizuka
• 通讯作者: Toru Ishizuka

The fate-determinants of adult newborn neurons : monitoring the caspase activities and optogenetic control at single-cell level

成年新生神经元的命运决定因素：单细胞水平的半胱天冬酶活性监测和光遗传学控制

• 发表时间: 2011
• 作者: Jun Yokose;Toru Ishizuka;Hiromu Yawo
• 通讯作者: Hiromu Yawo

Lineage analysis of newly generated neurons in organotypic culture of rat hippocampus

• DOI: 10.1016/j.neures.2010.11.010
• 发表时间: 2011-03-01
• 期刊: NEUROSCIENCE RESEARCH
• 影响因子: 2.9
• 作者: Yokose, Jun;Ishizuka, Toru;Yawo, Hiromu
• 通讯作者: Yawo, Hiromu

Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector

使用基于 DMD 的投影仪对大脑切片中的神经元进行并行和图案化光遗传学操作

• DOI: 10.1016/j.neures.2012.03.009
• 发表时间: 2012
• 期刊: Neuroscience Research
• 影响因子: 2.9
• 作者: Sakai S;Ueno K;Ishizuka T;Yawo H
• 通讯作者: Yawo H