Bioimaging of tumor markers using both engineered lectins and antibodies

使用工程凝集素和抗体对肿瘤标志物进行生物成像

• 批准号: 21390173
• 负责人: YAMAMOTO Kazuo
• 金额: $ 12.48万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390173/
• 关键词: レクチン; 腫瘍マーカー; がん糖鎖抗原; イメージング

It is crucial to identify biomarkers that are highly selective and sensitive in the diagnosis of cancers. We aimed to make a diagnosis platform using both antibodies and engineered lectins for detecting increased glycoprotein expression and alteration of sugar moieties accompanied with tumor progression, respectively. Two kinds of methods were applied to screen engineered lectins from randomly substituted lectin library, and several clones specific for tumor-associated glyco-antigens were obtained. Some cancer cells and specimens clearly stained with Fc-fusion proteins of these engineered lectins, which indicated that it would be a selective tool for diagnosis along with a specific antibody.

识别在癌症诊断中具有高度选择性和敏感性的生物标志物至关重要。我们的目的是使用抗体和工程凝集素建立一个诊断平台，分别检测伴随肿瘤进展的糖蛋白表达增加和糖部分的改变。应用两种方法从随机置换的凝集素文库中筛选工程凝集素，获得了多个肿瘤相关糖抗原特异性克隆。一些癌细胞和样本明显被这些工程凝集素的 Fc 融合蛋白染色，这表明它将与特定抗体一起成为诊断的选择性工具。

项目成果

Tumor cells of non-haematopoietic and haematopoietic origins express acivation-induced C-type lectin, the ligand for killer cell lectin-like receptor Fl.

非造血和造血来源的肿瘤细胞表达激活诱导的C型凝集素，其是杀伤细胞凝集素样受体F1的配体。

• 发表时间: 2010
• 期刊: Int.Immunol.
• 作者: Akatsuka A.;et al
• 通讯作者: et al

Huaman XTP3-B binds to al-antitrypsin variant null Hong Kong via the C-terminal MRH domain in a glycan-dependent manner

Huaman XTP3-B 通过 C 端 MRH 结构域以聚糖依赖性方式与 al-抗胰蛋白酶变体 null Hong Kong 结合

• 发表时间: 2010
• 期刊: Glycobiology 20
• 作者: Yamaguchi D.;et al.
• 通讯作者: et al.

The sugar-binding ability of human OS-9 and its involvement in ER-associated degradation

• DOI: 10.1093/glycob/cwp175
• 发表时间: 2010-03-01
• 期刊: GLYCOBIOLOGY
• 影响因子: 4.3
• 作者: Mikami, Kaoru;Yamaguchi, Daisuke;Yamamoto, Kazuo
• 通讯作者: Yamamoto, Kazuo

The sugar-binding ability of human OS-9 and its involvment in ER-associated degradation

人 OS-9 的糖结合能力及其参与 ER 相关降解

• 发表时间: 2010
• 期刊: Glycobiology 20
• 作者: Mikami K.;et al.
• 通讯作者: et al.

レクチン提示細胞、レクチンライブラリー、及びレクチンのスクリーニング方法

凝集素呈递细胞、凝集素文库和凝集素筛选方法

• 发表时间: 2012