The physiological function of the quantitative traits loci for the traits associated with the strong culm to develop the rice cultivars with high grain yield and biomass production.

强秆相关性状数量性状基因座的生理功能对培育高产、高生物量水稻品种的作用.

基本信息

批准号：
21380013
负责人：
OOKAWA Taiichiro
金额：
$ 11.65万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

Lodging resistance is one of the most important agronomical traits for the rice cultivars with high grain yield and biomass production. To locate the chromosome regions controlling the lodging resistance in rice, we conducted to estimate and characterize the quantitative traits loci(QTLs) responsible for culm mechanical strength using QTL analyses, using a set of backcross inbred lines(BILs) and the chromosome segment substitution lines(SLs) developed from a cross between Koshihikari, and a high lodging resistant cultivars. We identified the effective quantitative trait loci(QTLs) for culm strength, which were designated STRONG CULM2(SCM2) and STRONG CULM3(SCM3). Positional cloning of the gene revealed that SCM2 was identical to APO1, a gene previously reported to control panicle structure. A near-isogenic line carrying SCM2 showed enhanced culm strength and increased spikelet number due to the pleiotropic effects of the gene. Although SCM2 is a gain-of-function mutant of APO1, it does not have the negative effects reported for APO1 over-expression mutants, such as decreased panicle number and abnormal spikelet morphology. The identification of lodging resistance genes by QTL analysis combined with positional cloning is a useful approach for improving lodging resistance and overall productivity in rice.

耐药性是具有高晶粒产量和生物量产生的水稻品种最重要的农艺特征之一。为了找到控制大米中住宿耐药性的染色体区域，我们使用QTCROSS近交线（BIL）和从sls sepriention Liverration Liverrortion Liverrars（SLS）之间开发了kirihihikihikari and和soshihikari and and和soshikari and and and and and and and and and and and and and和soshihikari，估算和表征了负责使用QTL分析来负责CULM机械强度的定量性状基因座（QTL）。我们确定了用于CULM强度的有效定量性状基因座（QTL），这些定量位点被指定为强CULM2（SCM2）和强CULM3（SCM3）。该基因的位置克隆表明SCM2与APO1相同，APO1相同，该基因先前据报道控制圆锥花序结构。携带SCM2的近乎异源线显示出增强的CULM强度和由于基因的多效效应而增加的尖峰数量。尽管SCM2是APO1的功能收益突变体，但对APO1过表达突变体的负面影响没有报道的负面影响，例如圆锥花序数量降低和异常的尖峰形态。通过QTL分析与位置克隆结合鉴定住宿抗性基因是提高水稻中耐药性和整体生产力的有用方法。