Design of Molecular Assembly for Extraction of Biomolecules

生物分子提取的分子组装设计

• 批准号: 21360383
• 负责人: GOTO Masahiro
• 金额: $ 11.4万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21360383/
• 关键词: 逆ミセル; 溶媒抽出; マイクロエマルション; 分子集合体; タンパク質; DNA; 抽出; 分子認識; DNA抽出; タンパク質分離; イオン交換; 液液抽出; 分離操作; イオン液体; タンパク質抽出; 高度分離; 逆キセル; 分離

Liquid/liquid extraction is one of the useful separation techniques involving a molecular recognition event. Despite a number of studies on liquid/liquid extraction, it is still a challenge to extract a target biomolecule selectively to another phase based on the precise recognition at a liquid/liquid interface. In particular, if the target is a biomacromolecule, the presence of multifunctional groups prevents selectivity and versatility in liquid/liquid extraction. We here propose the sequence-specific liquid/liquid extraction of DNA oligonucleotides using reverse micelles with a DNA-surfactant. A reverse micelle, which is a nanoscale water pool surrounded by surfactant molecules in an organic solvent, is one of the highly useful tools for extraction of biomolecules.

液/液萃取是涉及分子识别事件的有用分离技术之一。尽管对液/液萃取进行了大量研究，但基于液/液界面的精确识别将目标生物分子选择性地萃取到另一相仍然是一个挑战。特别是，如果目标是生物大分子，则多功能基团的存在会妨碍液/液萃取的选择性和多功能性。我们在此提出使用带有 DNA 表面活性剂的反胶束对 DNA 寡核苷酸进行序列特异性液/液提取。反胶束是有机溶剂中被表面活性剂分子包围的纳米级水池，是提取生物分子的非常有用的工具之一。

项目成果

Activation and stabilization of enzymes in ionic liquids

• DOI: 10.1039/b926130c
• 发表时间: 2010-01-01
• 期刊: ORGANIC & BIOMOLECULAR CHEMISTRY
• 影响因子: 3.2
• 作者: Moniruzzaman, Muhammad;Kamiya, Noriho;Goto, Masahiro
• 通讯作者: Goto, Masahiro

Design of a cytochrome P450BM3 reaction system linked by two-step cofactor regeneration catalyzed by a soluble transhydrogenase and glycerol dehydrogenase

可溶性转氢酶和甘油脱氢酶催化的两步辅因子再生连接的细胞色素P450BM3反应系统的设计

• 发表时间: 2009
• 期刊: Biotechnol. Prog
• 作者: T. Mouri;T. Shimizu;N. Kamiya;H. Ichinose;M. Goto
• 通讯作者: M. Goto

Preparation of Lactose-Modified Cellulose Films by a Nonaqueous Enzymatic Reaction and their Biofunctional Characteristics as a Scaffold for Cell Culture

• DOI: 10.1021/bm900089j
• 发表时间: 2009-05-01
• 期刊: BIOMACROMOLECULES
• 影响因子: 6.2
• 作者: Esaki, Kei;Yokota, Shingo;Wariishi, Hiroyuki
• 通讯作者: Wariishi, Hiroyuki

Biocatalysis in Water-in-Ionic Liquid Microemulsions

离子液体包水微乳液中的生物催化

• 发表时间: 2009
• 期刊: Langmuir
• 影响因子: 3.9
• 作者: M. Moniruzzaman;N. Kamiya;M. Goto
• 通讯作者: M. Goto

Transglutaminase-mediated protein labeling with a designed peptide loop

使用设计的肽环进行转谷氨酰胺酶介导的蛋白质标记

• 发表时间: 2011
• 期刊: Biochem. Biophys. Res. Commun
• 作者: Y. Mori;M. Goto;N. Kamiya
• 通讯作者: N. Kamiya