Analysis of the mechanisms of the synapse formation of newborn neurons by developing mice with channelrhodopsin-2 only in newborn neuron in the dentate gyrus.

通过仅在齿状回新生神经元中使用通道视紫红质2的小鼠来分析新生神经元突触形成的机制。

基本信息

批准号：
21200016
负责人：
YASUDA Hiroki
金额：
$ 19.8万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

We planned to develop a new genetically modified mice whose newborn neurons in the dentate gyrus in the hippocampus posess channelrhodopsin-2 (chr2) to analyze the mechanisms of synapse formation of newborn neurons. We are still in the middle of generating the chr2 mice we originally planned, instead we used another mice in which neurons with markers of inhibitory cells express channelrhodopsin-2, because newborn excitatory neurons in the dentate gyrus also have these markers until about three weeks after birth. We now check whether light stimulation on the dentate gyrus evoked EPSCs in pyramidal neurons in the CA3 region and inhibitory neurons in the hilus.Recent literature suggests that antidepressants improve depressed mood through increasing generation of newborn neurons in the dentate gyrus in the hippocampus, therefore we examined the effects of stress, which can cause depression, on the excitability of granule cells in the dentate gyrus. We found that forced swimming made granule neurons more excitable and now investigating the mechanisms that underlie the elevated excitability.

我们计划开发一种新的转基因小鼠，其海马齿状回的新生神经元具有通道视紫红质2（chr2），以分析新生神经元突触形成的机制。我们仍在生产最初计划的 chr2 小鼠，相反，我们使用了另一只小鼠，其中具有抑制细胞标记的神经元表达视紫红质通道蛋白-2，因为齿状回中的新生兴奋性神经元直到大约三周后也具有这些标记。出生。我们现在检查齿状回的光刺激是否会诱发 CA3 区锥体神经元和门部抑制性神经元中的 EPSC。最近的文献表明，抗抑郁药通过增加海马齿状回新生神经元的生成来改善抑郁情绪，因此我们研究了可导致抑郁的压力对齿状回颗粒细胞兴奋性的影响。我们发现强迫游泳使颗粒神经元更加兴奋，现在正在研究兴奋性升高的机制。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Identification of the Cluster Control Region for the Protocadherin-β Genes Located beyond the Protocadherin-γ Cluster.

位于原钙粘蛋白-γ 簇之外的原钙粘蛋白-β 基因的簇控制区的鉴定。

DOI：
发表时间：
2011
期刊：
J. Biol. Chem.
影响因子：
0
作者：
Shinnichi Yokota;Teruyoshi Hirayama;Keizo Hirano;Ryosuke Kaneko;Shunsuke Toyoda;Yoshimi Kawamura;Masumi Hirabayashi;Takahiro Hirabayashi and Takeshi Yagi
通讯作者：
Takahiro Hirabayashi and Takeshi Yagi

Frequent spontaneous seizures followed by spatial working memory/anxiety deficits in mice lacking sphingosine 1-phosphate receptor 2