Regulation mechanisms of host cell cycle by baculovirus infection

杆状病毒感染对宿主细胞周期的调控机制

• 批准号: 13660059
• 负责人: IKEDA Motoko
• 金额: $ 2.3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660059/
• 关键词: baculovirus; AcMNPV; Sf9 cell; cell cycle; apoptosis; 核多角体病ウイルス

Infection of Autographa californica M nucleopolyhedrovirus (AcMNPV) in Sf9 cells induces cell cycle arrest at S and G2/M phases. To understand the mechanisms of the cell cycle arrest, identification of virus genes responsible for cell cycle arrest was performed. And also changing patterns of several cellular factors responsible for cell cycle regulation were analyzed in Sf9 cells during AcMNPV infection by using several antibodies against those homologues in other animals.

Sf9 细胞中苜蓿银纹夜蛾 M 核多角体病毒 (AcMNPV) 的感染会诱导细胞周期停滞在 S 期和 G2/M 期。为了了解细胞周期停滞的机制，对负责细胞周期停滞的病毒基因进行了鉴定。通过使用针对其他动物中这些同源物的几种抗体，还分析了 AcMNPV 感染期间 Sf9 细胞中负责细胞周期调节的几种细胞因子的变化模式。