Study on novel tissue regenerative therapy using bone marrow-derived mesenchymal stem cells and FGF/Phosphophoryn/Collagen complex

利用骨髓间充质干细胞和 FGF/磷酸化/胶原复合物进行新型组织再生疗法的研究

• 批准号: 21592630
• 负责人: FUJII Takeo
• 金额: $ 2.91万
• 依托单位: Matsumoto Dental University (2011)Health Sciences University of Hokkaido (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592630/
• 关键词: 歯周外科学; 再生治療; 三次元培養系; 歯髄間葉系幹細胞; ホスホホリン; FGF; 再生医療; 歯周組織再生; 骨髄間葉系幹細胞

With the goal to establish new tissue regenerative therapy for extensive regeneration of destroyed periodontal tissues accompanying vertical or horizontal bone defects due to severe periodontitis, The aim of this study was to evaluate the kinetics of bone marrow-derived mesenchymal stem cells cultured in a FGF. collagen complex as a regenerative scaffold in vitro. Bone marrow-derived mesenchymal stem cells showed good growth in the collagen gel culture. The effect of FGF in promoting cell growth is exhibited at the early stage of culture, and the ALP activity has already passed the peak when assayed at 14 days of culture at FGF concentrations of 10 ng/ml. The ALP activity showed in the presence of phosphophoryn in a dose-dependent manner at 14 days of culture. A marked difference in calcium content was not observed. The present study sheds light on the development of an optimal biomaterial that forms the basis of calcified hard tissue formation, suggesting the feasibility of applying the 3-D collagen gel cell culture system for the construction of a scaffold that controls the amount of regenerating tissue.

为了建立新的组织再生疗法，以通过严重的牙周炎引起的垂直或水平骨缺损的破坏牙周组织的广泛再生，这项研究的目的是评估在FGF中培养的骨髓衍生的间质干细胞的动力学。胶原蛋白复合物在体外作为再生支架。骨髓衍生的间充质干细胞在胶原蛋白培养中表现出良好的生长。 FGF在培养的早期阶段表现出FGF在促进细胞生长方面的影响，当在FGF浓度为10 ng/mL的FGF浓度时，ALP活性已经通过了峰值。 ALP活性在培养物14天的磷脂中以剂量依赖性方式显示。未观察到钙含量的明显差异。本研究阐明了构成钙化硬组织形成基础的最佳生物材料的发展，这表明将3-D胶原凝胶细胞培养系统应用于控制再生组织量的脚手架的可行性。

项目成果

Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients

FGF-2在牙周炎患者中应用的牙周组织再生

• 发表时间: 2010
• 作者: T.Fujii;Y.Terada;M.Akamatsu;M.Watanuki
• 通讯作者: M.Watanuki

Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients.

FGF-2 在牙周炎患者中的应用实现牙周组织再生。

• 发表时间: 2009
• 期刊: J Clin Periodontol 36
• 作者: T.Fujii;Y.Terada;M.Akamatsu;M.Watanuki.
• 通讯作者: M.Watanuki.

Periodontal Tissue Regeneration by the Application of FGF-2 in Patients

FGF-2在患者中应用的牙周组织再生

• 发表时间: 2010
• 作者: T. Fujii;Y. Terada;M. Akamatsu;M. Watanuki
• 通讯作者: M. Watanuki

Murakami FGF-2 stimulates periodontal regeneration : results of multicenter randomized clinical trial

Murakami FGF-2 刺激牙周再生：多中心随机临床试验结果

• 发表时间: 2011
• 期刊: J Dent Res
• 影响因子: 7.6
• 作者: Kitamura M;Akamatsu M;Machigashira M;Hara Y;Sakagami R;Hirofuji T;Hamachi T;Maeda K;Yokota M;Kido J;Nagata T;Kurihara H;Takashiba S;Sibutani T;Fukuda M;Noguchi T;Yamazaki K;Yoshie H;Ioroi K;Arai T;Nakagawa T;Ito K;Oda S;Izumi Y;Oga
• 通讯作者: Oga

FGF-2 stimulates periodontal regeneration : results of multicenter randomized clinical trial.

FGF-2 刺激牙周再生：多中心随机临床试验的结果。

• 发表时间: 2011
• 期刊: J Dent Res.
• 作者: Kitamura M;Akamatsu M,Fujii;T;et al.
• 通讯作者: et al.