Analysis of epithelial integrity system in tumor invasion and metastasis of oral cancer

口腔癌肿瘤侵袭转移中上皮完整性系统分析

• 批准号: 21592526
• 负责人: HIGASHIKAWA Koichiro
• 金额: $ 2.83万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592526/
• 关键词: 口腔癌; 上皮間葉移行; 癌の浸潤・転移; p^63; 上皮整合性; 上皮・間葉移行; p63

Epithelial-Mesencymal Transition(EMT) is involved in tumor invasion and metastasis of oral squamous cell carcinomas(SCCs). Snail, a transcription factor and EMT inducer, directs transcription of its target genes for the acquisition of EMT in SCC cells. We focused a process of loss of epithelial integrity among SCC cells during the EMT. For the investigation of that, we generated SCC cell pools of forced Snail expression by recombinant virus infection system. Almost the cells displayed constant expression of exogenous Snail, however, a small population of the cells underwent EMT. The cells on culture dish were given the migration area and were escalated into EMT. Immnocytochemistory and immnoblot analyses showed that loss of epithelial integrity and cell-cell contact were the last step of EMT process and the process was accelerated by distinct humoral factors.

上皮间质转化（EMT）参与口腔鳞状细胞癌（SCC）的肿瘤侵袭和转移。 Snail 是一种转录因子和 EMT 诱导剂，指导其靶基因的转录，以在 SCC 细胞中获得 EMT。我们重点关注 SCC 细胞在 EMT 过程中上皮完整性丧失的过程。为了对此进行研究，我们通过重组病毒感染系统生成了强制 Snail 表达的 SCC 细胞池。几乎所有细胞都持续表达外源性 Snail，然而，一小部分细胞经历了 EMT。培养皿上的细胞被给予迁移区域并升级为EMT。免疫细胞化学和免疫印迹分析表明，上皮完整性和细胞间接触的丧失是 EMT 过程的最后一步，并且不同的体液因子会加速该过程。

项目成果

Paracrine PGE2 targets epiregulin-overexpressing squamous cell carcinoma for resistance to anti-5-FU-induced apoptosis

旁分泌 PGE2 靶向上皮调节蛋白过度表达的鳞状细胞癌，以抵抗抗 5-FU 诱导的细胞凋亡

• 发表时间: 2010
• 作者: Kitazoe K;Park YS;Kaji N;Okamoto Y;Tokeshi M;Kogure K;Harashima H;Baba Y;熊谷泉;東川浩一郎
• 通讯作者: 東川浩一郎

Functional analysis of Snail-mediated downregulation of deltaNp63alpha in squamous cell carcinoma cells

Snail 介导的鳞状细胞癌细胞中 deltaNp63alpha 下调的功能分析

• 发表时间: 2009
• 作者: 東川晃一郎;他
• 通讯作者: 他

Overexpression of the receptor for hyaluronan-mediated motility, correlates with expression of microtubule-associated protein in human oral squamous cell carcinomas

• DOI: 10.3892/ijo_00000286
• 发表时间: 2009-06-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Shigeishi, Hideo;Fujimoto, Shinichi;Kamata, Nobuyuki
• 通讯作者: Kamata, Nobuyuki

Lymphangiogenesis and metastasis : Role of tumor-inducedlymphatic vessel activation in extramammary Paget' s disease

淋巴管生成和转移:肿瘤诱导的淋巴管激活在乳房外佩吉特病中的作用

• 发表时间: 2009
• 期刊: Am JPathol
• 作者: Hirakawa S;Detmar M;Kerjaschki D;Nagamatsu S;Matsuo K;Tanemura A;Kamata N;Higashikawa K;Okazaki H;Kameda K;Nishida-Fukuda H;Mori H;Hanakawa Y;Sayama K;Shirakata Y;Tohyama M;Tokumaru S;Katayama I;Hashimoto K
• 通讯作者: Hashimoto K

PGE(2) targets squamous cell carcinoma cell with the activated epidermal growth factor receptor family for survival against 5-fluorouracil through NR4A2 induction

PGE(2) 以激活的表皮生长因子受体家族为目标，通过 NR4A2 诱导，针对 5-氟尿嘧啶存活的鳞状细胞癌细胞

• 发表时间: 2011
• 期刊: Cancer Lett
• 影响因子: 9.7
• 作者: Yuji Kawai;Yukie Nagai;Minoru Asada;Nguyen PT;○Yoshio Okahata and Hiroyuki Furusawa;Shigeishi H
• 通讯作者: Shigeishi H