Evaluations of fibroblast apoptosis in the stress-deprived patellar tendon in vivo

体内应力剥夺髌腱成纤维细胞凋亡的评价

• 批准号: 21591927
• 负责人: KITAMURA Nobuto
• 金额: $ 2.83万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591927/
• 关键词: 腱・靭帯組織; 除負荷; アポト-シス; 健・靭帯組織; アポトーシス

The effect of stress deprivation on the tendon tissue has been an important focus in the field of biomechanics. Rabbits were divided into stress-shielded, sham-operated, and control groups. To completely shield the patellar tendon from stress, we used an established surgical method. Both the number and the ratio of TUNEL-, caspase-3-, JNK-, and p38-positive cells were significantly greater (p<0.0001) in the stress-shielded group than in the sham group at 24h, 2, and 4 days. This study demonstrated that complete stress deprivation induces fibroblast apoptosis in vivo with activation of JNK and p38 within 24h. This fact suggested that the fibroblast apoptosis caused by stress deprivation is induced via the mitogen-activated protein kinase signaling pathway.

应力剥夺对肌腱组织的影响一直是生物力学领域的一个重要焦点。将兔子分为应激屏蔽组、假手术组和对照组。为了完全保护髌腱免受压力，我们使用了既定的手术方法。 24小时、2天和4天时，应激屏蔽组中TUNEL-、caspase-3-、JNK-和p38阳性细胞的数量和比例均显着高于假手术组（p<0.0001） 。这项研究表明，完全的应激剥夺会在 24 小时内诱导体内成纤维细胞凋亡，并激活 JNK 和 p38。这一事实表明，应激剥夺引起的成纤维细胞凋亡是通过丝裂原激活蛋白激酶信号通路诱导的。

项目成果

膝屈筋腱-ハイブリッド代用材料を用いた1 束前十字靱帯再建後と解剖学的2重束前十字靱帯再建後のX線学的骨孔径変化の比較

膝屈肌腱混合替代材料单束前交叉韧带重建与解剖双束前交叉韧带重建后X线骨孔直径变化的比较

• 发表时间: 2010
• 作者: 河口泰之;北村信人;近藤英司;松田英敏;稲垣有佐;横田正司;北山総一郎;小野寺純;今淵隆誠;甲斐秀顯;宮武慎;小野寺伸;遠山晴一;安田和則;井上雅之
• 通讯作者: 井上雅之

Biomechanical Evaluation of a Novel Application of a Fixation Device for Bone-Tendon-Bone Graft (EndoButton CL BTB) to Soft-Tissue Grafts in Anatomic Double-Bundle Anterior Cruciate Ligament Reconstruction

• DOI: 10.1016/j.arthro.2010.01.007
• 发表时间: 2010-09-01
• 期刊: ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
• 影响因子: 4.7
• 作者: Miyatake, Shin;Kondo, Eiji;Yasuda, Kazunori
• 通讯作者: Yasuda, Kazunori

The effect of autologous meniscal fragment I implantation on meniscus regeneration results from synergic effects of meniscal matrix and living chondrocyte implantations

自体半月板碎片I植入对半月板再生的影响源于半月板基质和活体软骨细胞植入的协同作用

• 发表时间: 2012
• 作者: Kamaguchi Y;Kondo E;Kitamura N;el;al.
• 通讯作者: al.

マクロファ-ジ遊走阻止因子遺伝子の欠損が膝内側側副靭帯損傷の治癒に与える影響

巨噬细胞迁移抑制因子基因缺失对膝关节内侧副韧带损伤愈合的影响

• 发表时间: 2010
• 作者: 北山聡一郎;小野寺伸;宮武慎;近藤英司;北村信人;遠山晴一;安田和則
• 通讯作者: 安田和則

Sress deprivation from the patellar tendon induces apoptosis of fibroblasts in vivo with activation of mitogen-activated protein kinases

髌腱应力剥夺会通过丝裂原激活蛋白激酶的激活诱导体内成纤维细胞凋亡

• 发表时间: 2009
• 期刊: J Biomech
• 影响因子: 2.4
• 作者: Kawabata H;Katsura T;Kondo E;Miyatake S;Tanabe Y;Kitamura N;Setoguchi T;Komiya S;Yasuda K
• 通讯作者: Yasuda K