The efficacy of anti-Aβoligomer antibody to the toxicity of Aβto neuronal cells

抗Aβ寡聚体抗体对抗Aβ对神经元细胞毒性的功效

• 批准号: 21591524
• 负责人: HAYASHI Yoshihito
• 金额: $ 3万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591524/
• 关键词: アルツハイマー型認知症; βアミロイド; オリゴマー; 神経細胞保護

The most popular type of dementia is Alzheimer's disease(AD). The cause of AD is thought that amyloid beta protein(Aβ) cleaved from amyloid precursor protein(APP) which is expressing on the neuronal cell membranes has neurotoxicity and induce neuronal cell death. Recently some researchers found the soluble oligomer form had toxicity to the synapses and induce cognitive decline. We thought that anti-oligomer antibody has neuroprotective effect. We investigated the mechanisms of neuroprotective effect of anti-oligomer antibody against neuronal cells from Aβtoxicity. We investigated whether or not anti-oligomer antibody reduce the amount of free radical formation when Aβaggregate themselves. The amount of free radical formation was reduced compared with control solution. The viability of PC12 cells was higher when culture medium was added anti-oligomer antibody with Aβ. We found some changes of amount of NMDA receptor in PC12 when added Aβto culture medium with anti-oligomer antibody. We did not confirm whether the effect of anti-oligomer antibody against NMDA receptor was direct or indirect effect. We thought that there are some relations between two properties.

最常见的痴呆类型是阿尔茨海默氏病（AD），AD的病因被认为是从神经元细胞膜上表达的淀粉样前体蛋白（APP）裂解而来的淀粉样β蛋白（Aβ）具有神经毒性并诱导神经元细胞死亡。最近一些研究人员发现可溶性寡聚体形式对突触具有毒性并导致认知能力下降，我们认为抗寡聚体抗体具有神经保护作用，我们研究了其神经保护作用的机制。抗寡聚体抗体对神经元细胞的Aβ毒性，无论是否研究，当Aβ自身聚集时，抗寡聚体抗体减少了自由基形成量，与对照溶液相比，PC12细胞的活力更高。当培养基中添加抗寡聚体抗体时，我们发现PC12中NMDA受体的量发生了一些变化，但我们没有确认是否有影响。抗寡聚体抗体对NMDA受体有直接或间接的作用，我们认为这两种特性之间存在一定的关系。

项目成果

Effects of Yokukansan on behavioral and psychological symptoms of dementia in regular treatment for Alzheimer's disease

• DOI: 10.1016/j.pnpbp.2010.02.013
• 发表时间: 2010-04-16
• 期刊: PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
• 影响因子: 5.6
• 作者: Okahara, Kazunori;Ishida, Yasushi;Mitsuyama, Yoshio
• 通讯作者: Mitsuyama, Yoshio

アルツハイマー型認知症の周辺症状(BPSD)に対する抑肝散の効果-日常診療下における検討:入院患者における部分集団解析-

抑肝散对阿尔茨海默病（BPSD）周围症状的影响 - 日常临床实践检查：住院患者的亚组分析 -

• 发表时间: 2010
• 期刊: Geriatric Medicine(老年医学)
• 作者: 林要人;石田康;井上輝彦;宇田川充隆;竹内康三;木上幸一郎;二宮嘉正;河野次郎;鮫島哲郎;川原岳;後藤勇;首藤謙二;倉山茂樹;中村純五;岡原一徳;三山吉夫
• 通讯作者: 三山吉夫

A multicenter open label study to investigate the effect of a traditional Japanese medicine, yokukansan, on the behavioral and psychological symptoms of dementia in patients with Alzheimer's disease

一项多中心开放标签研究，旨在调查日本传统药物“抑肝散”对阿尔茨海默病患者痴呆行为和心理症状的影响

• 发表时间: 2009
• 作者: Yoshihito Hayashi;Y Mitsuyama;Y Ishida
• 通讯作者: Y Ishida

Effect of aripiprazole on methamphetamine-induced disruption of latent inhibition in rats

阿立哌唑对甲基苯丙胺诱导的大鼠潜伏抑制破坏的影响

• 发表时间: 2011
• 期刊: J Behav Brain Sci
• 作者: Matsuo H;Abe H;Ikeda T;Ebihara K;Takeda R;Nishimori T;Ishida Y
• 通讯作者: Ishida Y

オレキシンAによる脳内自己刺激閾値の上昇はCRF受容体阻害によって抑制される

CRF 受体抑制可抑制食欲素 A 诱导的大脑自我刺激阈值的增加

• 发表时间: 2009
• 作者: 畑敏道;蛯原功介;伊達紫;石田康;中原大一郎
• 通讯作者: 中原大一郎