Analysis of the effect of visible light on photoaged skin focusing on DNA repair

分析可见光对光老化皮肤的影响，重点关注DNA修复

基本信息

批准号：
21591475
负责人：
MORIWAKI Shinichi
金额：
$ 2.91万
依托单位：
Osaka Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

Visible light(VL) such as intense pulsed light(IPL) and light emitting diodes(LED) have been recently introduced to the painless, nonabrasive phototherapy to reverse photoaging skin such as wrinkles and lentigines. However the precise basic mechanisms on which VL irradiation can modulate these aged skin changes has not yet been proven. Here, we examined the effect of LED on cell proliferation, production of hyaluronic acid, DNA repair ability and melanogenesis by use of the human primary cultured fibroblasts and the 3-D skin-like tissue structure. A rate of cell proliferation and production of hyaluronic acid from the cells were significantly increased after red and blue LED irradiation. In addition, red, yellow and blue LED could stimulate the ability to repair oxidative DNA damage including 8-oxo-G of the cells assessed by host cell reactivation of the reporter plasmid. Melanogenesis was inhibited on the human 3-D skin-like tissue exposed to red or blue LED without no histologically damage of the skin. These findings suggest that LED light can be useful for skin rejuvenation through mechanisms of activating dermal skin fibroblast, increasing DNA repair ability to oxidative DNA damage and suppressing melanogenesis.

最近已将可见光（VL）（VL）（IPL）和发光二极管（LED）引入了无痛的，非散热的光疗，以逆转光照的皮肤，例如皱纹和凸齿。但是，VL辐射可以调节这些老化皮肤变化的确切基本机制尚未得到证明。在这里，我们通过使用人类原代培养的成纤维细胞和3-D皮肤样组织结构来研究LED对细胞增殖，透明质酸的产生，DNA修复能力和黑色素生成的影响。红色和蓝色LED辐照后，细胞增殖的速率和透明质酸产生的速率显着增加。此外，红色，黄色和蓝色LED可以刺激修复氧化性DNA损伤的能力，包括通过报告质粒的宿主细胞重新激活评估的8-氧基-G。黑色素生成被抑制在人类3-D皮肤的组织上，暴露于红色或蓝色LED的情况下，没有任何组织学损害皮肤。这些发现表明，通过激活皮肤皮肤成纤维细胞的机制，LED光可以对皮肤复兴有用，从而提高DNA修复能力以氧化DNA损伤和抑制黑色素生成。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Experience of laboratory diagnosis of XP

XP实验室诊断体会

DOI：
发表时间：
2010
期刊：
影响因子：
0
作者：
Song N;Boku S;Nakagawa S;Kato A;Toda H;Takamura N;Omiya Y;Kitaichi Y;Inoue T;Koyama T;Teraishi M;Moriwaki S
通讯作者：
Moriwaki S

Effects of red and blue light emitting diode (LED) on the cultured human skin

红光和蓝光发光二极管 (LED) 对培养人体皮肤的影响