Roll of skin antioxidant system in UV irradiation and epidermal morphogenesis/differentiation

皮肤抗氧化系统在紫外线照射和表皮形态发生/分化中的作用

• 批准号: 21591454
• 负责人: KAWACHI Yasuhiro
• 金额: $ 2.91万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591454/
• 关键词: 酸化ストレス; Nrf2; ケラチノサイト; 紫外線; 皮膚老化

Ultraviolet (UV) radiation is one of the most important environmental factors involved in the pathogenesis of premature skin aging, termed photoaging. The harmful effects of UV in photoaging are associated with the generation of reactive oxygen species, and cellular antioxidants act to prevent the occurrence and reduce the severity of UV-induced photoaging. The transcription factor Nrf2 and its cytoplasmic anchor protein, Keap1, are central regulators of the cellular antioxidant response. Here, we investigated the role of the Nrf2. Keap1 pathway in photoaging using nrf2 gene-deficient (nrf2-/-) mice. Our results indicated that UVB-irradiated nrf2-/-mice showed accelerated photoaging, such as coarse wrinkle formation, loss of skin flexibility, epidermal thickening, and deposition of extracellular matrix in the upper dermis. In addition, nrf2-/-mice also showed an increase in cutaneous reactivity for the lipid peroxidation product 4-hydroxy-2-nonenal and a significant decrease in cutaneous glutathione level. These findings indicate that Nrf2 plays the important role in the protection against UVB-induced photoaging.

紫外线（UV）辐射是过早皮肤老化的发病机理所涉及的最重要的环境因素之一，称为光照。紫外线在光学中的有害作用与活性氧的产生有关，细胞抗氧化剂可防止发生这种情况并降低紫外线诱导的光学的严重程度。转录因子NRF2及其细胞质锚固蛋白KEAP1是细胞抗氧化剂反应的中心调节剂。在这里，我们研究了NRF2的作用。 Keap1使用NRF2基因缺陷型（NRF2 - / - ）小鼠光照。我们的结果表明，UVB辐射的NRF2 - / - 小鼠显示出加速的光照，例如粗皱纹形成，皮肤柔韧性的丧失，表皮增厚和上层皮肤外细胞外基质的沉积。此外，NRF2 - / - 小鼠还显示出脂质过氧化产物4-羟基-2-非烯醇的皮肤反应性增加，皮肤谷胱甘肽水平显着降低。这些发现表明，NRF2在防止UVB诱导的光学的保护中起着重要作用。

项目成果

Overexpression of the Transcription Factor Yin-Yang-1 Suppresses Differentiation of HaCaT Cells in Three-Dimensional Cell Culture

• DOI: 10.1038/jid.2010.229
• 发表时间: 2011-01-01
• 期刊: JOURNAL OF INVESTIGATIVE DERMATOLOGY
• 影响因子: 6.5
• 作者: Taguchi, Shijima;Kawachi, Yasuhiro;Otsuka, Fujio
• 通讯作者: Otsuka, Fujio

Extension of a malignant tailgut cyst to a subcutaneous space in the buttock.

恶性尾肠囊肿延伸至臀部皮下空间。

• 发表时间: 2010
• 期刊: Eur J Dermatol. 20(6)
• 作者: Fujisawa Y;Yanagisawa K;Nakamura Y;Kawachi Y;Otsuka F
• 通讯作者: Otsuka F

Superficial malignant peripheral nerve sheath tumor arising from a solitary cutaneous neurofibroma in a non-NF1 patient

非 NF1 患者中孤立性皮肤神经纤维瘤引起的浅表恶性周围神经鞘瘤

• 发表时间: 2011
• 期刊: Eur J. Dermatol
• 作者: Kawachi Y;Tanaka R;Fujisawa Y;Furuta J;Nakamura Y;Ishii Y;Otsuka F
• 通讯作者: Otsuka F

酸化ストレス応答因子Nrf2が皮膚の紫外線反応に果たす役割

氧化应激反应因子Nrf2在皮肤紫外线反应中的作用

• 发表时间: 2011
• 作者: Kawachi Y;Fujisawa Y;Furuta J;Nakamura Y;Ishii Y;Otsuka F;川内康弘
• 通讯作者: 川内康弘

Chronically recurrent and disseminated tinea faciei/corporis--autoinoculation from asymptomatic tinea capitis carriage.

慢性复发性和播散性面癣/体癣——无症状头癣携带者的自身接种。

• 发表时间: 2010
• 期刊: Pediatr Dermatol. 27(5)
• 作者: Kawachi Y;Ikegami M;Takase T;Otsuka F
• 通讯作者: Otsuka F