The Physiological function of milk gangliosides and their sialic acid containing polysaccharides : effect to the central nervous syptem ontogenesis and differentiation

乳神经节苷脂及其含唾液酸多糖的生理功能：对中枢神经系统个体发生和分化的影响

基本信息

批准号：
21591328
负责人：
IZUMI Tatsuro
金额：
$ 3万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

Objective : Gangliosides are present in high concentrations in the nervous tissue, and some are observed in small smounts in many extraneural tissues and body fluids. Human milk may play important roles in energy supplementation, prophylaxis of infection, and brain development. For preterm infants, human milk gangliosides are also very important substances during the early lactation stage. However, there are no data on human milk gangliosides from mothers at preterm delivery. We had described that human milk contains higher concentration of GD3 and GM3 gangliosides than cow's milk or infant milk formula. GD3 is a major component of the colostrums, and GM3 increases sharply at 8 d postpartum and then increases gradually in the milk of healthy human mothers with full-term delivery. We investigated the characterization of gangliosides and chronologic changes in human preterm milk earlier than 30 wk of gestation from 1 to 60 d after birth. And now, we also tried to examine the milk ganglio … More sides incorporation to the brain during suckling period.Methods : Forty-one samples were analyzed by high-performance thin-layer chromatography and a microtechnique using 1 mL of milk from each lactation and compared with 61 full-term human milk samples. After the preparation of ^<14>C-GD3 and ^<14>C-GM3, we tried to the incorporation of these gangliosides to the suckling rat brain, through milk.Results : Total lipid-bound sialic acid of human milk gangliosides after preterm delivery showed a peak concentration at 2 to 3 d postpartum and then remained at a high concentration until approximately 10 d. GD3 was the major ganglioside in the colostrum until approximately 7 to 10 d postpartum. GM3 was scarcely detected until 7 d postpartum and then increased gradually. There was no difference in the GD3 concentration per 1 mL of human milk between preterm and full-term human milk until approximately 5 to 8 d postpartum. After that time, the GD3 concentration decreased sharply. In contrast, the total concentrations of GM3 per 1 mL of human milk from mothers after preterm delivery were lower than those from mothers after full-term delivery throughout the entire period examined.Conclusion : This finding is essential to elucidate the composition of human milk gangliosides after preterm delivery, which may contribute to the analysis of the physiologic composition and formulation appropriate preterm infant nutrition.However, we could not confirm the incorporation of these gangliosides to the rat brain. Less

目的：神经毒剂存在于神经组织中的高浓度，其中一些在许多外部组织和体液中观察到一些小小的。人牛奶可能在补充能量，预防感染和大脑发育中起重要作用。对于早产儿，在早期泌乳阶段，人乳牛神经毒剂也是非常重要的物质。但是，在早产时，没有母亲的人牛奶神经毒剂的数据。我们描述的是，人类牛奶含有比牛奶或婴儿牛奶配方奶粉更高的GD3和GM3神经节苷脂。 GD3是初乳的主要组成部分，GM3在产后8 d时急剧增加，然后在健康的人类母亲的牛奶中逐渐增加。我们调查了在出生后1到60 d的30周妊娠30周的人类早产牛奶中神经节苷脂的表征。现在，我们还试图检查牛奶神经节……在哺乳期间，更多的侧面掺入了大脑。方法：通过高性能薄层色谱法分析了41个样品，并使用每种泌乳中的1 mL牛奶和61个全月的人奶样品分析了41个样品。在制备 ^<14> c-gd3和 ^<14> c-gm3之后，我们试图通过牛奶将哺乳后的大鼠掺入到哺乳后的大脑。乳糖：早产后，人牛奶神经毒剂的总脂质结合的唾液酸显示在2至3 d d的产后峰值浓度，然后在高浓度下升高到大约10 d d d d d d d d。 GD3是初乳中的主要神经节苷脂，直到产后7至10 d。直到产后7 d直到7 d才被检测到GM3，然后逐渐增加。在早产和足月牛奶之间，每1 mL人牛奶的GD3浓度没有差异，直到产后大约5至8 d。在那段时间之后，GD3浓度急剧下降。相比之下，早产后，每1 ml母乳中的GM3的总浓度低于母亲在整个期间的完整期间分娩后的母亲的总浓度。结论：判断对于阐明早产后的人类牛奶神经支出的组成至关重要脑。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Characterization and chronological changes of preterm human milk gangliosides

DOI：
10.1016/j.nut.2010.10.016
发表时间：
2011-10-01
期刊：
NUTRITION
影响因子：
4.4
作者：
Uchiyama, Shin-ichi;Sekiguchi, Kazuhito;Izumi, Tatsuro
通讯作者：
Izumi, Tatsuro

Detection of platelet-binding anti-measles and anti-rebulla virus lgG antibodies in infants with vaccine-induced thrombocytopenic purpura

疫苗诱导的血小板减少性紫癜婴儿血小板结合抗麻疹和抗风疹病毒IgG抗体的检测

DOI：
发表时间：
2011
期刊：
Vaccine
影响因子：
5.5
作者：
Okazaki N;Takeguchi N;Sonoda K;Handa Y;Kakiuchi T;Miyahara H;Akiyoshi K;Korematsu S;Suenobu S;Izumi T
通讯作者：
Izumi T

Novel mutation of early, perinatal anset myopathic type VLCAD deficiency

早期、围产期肌病型 VLCAD 缺陷的新突变