Analysis on molecular mechanisms of liver reconstitution by transplanted hepatic cells

肝细胞移植重建肝脏的分子机制分析

• 批准号: 21590833
• 负责人: AZUMA Seishin
• 金额: $ 2.91万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590833/
• 关键词: 肝臓学; 再生医学; 肝細胞; 幹細胞; 発生・分化; 肝細胞移植; CD13

Investigators of this research project have modulated the expression of target molecules of primary murine hepatic stem/progenitor cells purified by cell surface markers, and analyzed on the molecular mechanisms of liver repopulation by transplanted cells. We found that CD13 is a useful marker for enrichment of hepatic stem/progenitor cells. We also found that Sall4-mediated signaling pathway regulates the biliary differentiation of hepatic stem/progenitor cells. Our data indicated that modulation of MMP increases to the repopulation of recipient livers by donor cells. These studies contribute to the progress of autologous stem cell therapy against liver diseases.

该研究项目的研究人员通过细胞表面标记物调节纯化的原代小鼠肝干/祖细胞靶分子的表达，并分析移植细胞促进肝脏再生的分子机制。我们发现 CD13 是富集肝干/祖细胞的有用标记。我们还发现Sall4介导的信号通路调节肝干/祖细胞的胆管分化。我们的数据表明，MMP 的调节会增加供体细胞对受体肝脏的增殖。这些研究有助于自体干细胞治疗肝病的进展。

项目成果

Enrichment and Clonal Culture of Progenitor Cells During Mouse Postnatal Liver Development in Mice

• DOI: 10.1053/j.gastro.2009.06.001
• 发表时间: 2009-09-01
• 期刊: GASTROENTEROLOGY
• 影响因子: 29.4
• 作者: Kamiya, Akihide;Kakinuma, Sei;Nakauchi, Hiromitsu
• 通讯作者: Nakauchi, Hiromitsu

Treatment and outcomes for elderly patients with hepatocellular caricinoma

老年肝细胞癌患者的治疗和结局

• 发表时间: 2012
• 作者: Azuma S;Sakamoto N;Sakurai Y;Nakagawa M;Kakinuma S;Watanabe M
• 通讯作者: Watanabe M

マウス胎生中期肝幹/前駆細胞を用いた細胞移植

使用中胚小鼠肝干/祖细胞进行细胞移植

• 发表时间: 2009
• 作者: 柿沼晴;紙谷聡英;太田春彦;坂本直哉;渡辺守;中内啓光
• 通讯作者: 中内啓光

Inhibition of HCV replication by a specific inhibitor of serin-arginine-rich protein kinase

富含丝氨酸-精氨酸的蛋白激酶的特异性抑制剂抑制 HCV 复制

• 发表时间: 2010
• 期刊: Antimicrob Agent Chemother
• 作者: Karakama Y;Sakamoto N;Itsui Y;Nakagawa M;Tasaka-Fujita M;Nishimura-Sakurai Y;Kakinuma S;Oooka S;Azuma S;Tsuchiya K;Onogi H;Hagiwara M;Watanabe M.
• 通讯作者: Watanabe M.

肝臓細胞移植におけるドナー由来MatrixMetalloproteinaseの関与

供体来源的基质金属蛋白酶参与肝细胞移植

• 发表时间: 2010
• 作者: 柿沼晴;紙谷聡英;坂本直哉;渡辺守;中内啓光
• 通讯作者: 中内啓光