Identification of novel atopic dermatitis biomarkers by lipidomic analysis

通过脂质组学分析鉴定新型特应性皮炎生物标志物

• 批准号: 21590599
• 负责人: NAKAMURA Mitsuhiro
• 金额: $ 2.91万
• 依托单位: Gifu Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590599/
• 关键词: シグナル伝達; 脂質; アレルギー・喘息; 薬理学; 分析化学; アトピー性皮膚炎; バイオマーカー; 質量分析; アレルギー・ぜんそく

Sphingolipid metabolites such as ceramides, major lipids of the stratum corneum, are known to play critical roles in various epidermal functions. Patients with atopic dermatitis commonly show reduced ceramide contents of the skin.We developed several mass spectrometry methods(LC-MS/MS, MALDI-TOF, and LC/MS-IT-TOF) for the rapid, simultaneous quantification of C_<16>-, C_<18>-, C_<20>-, C_<22>-, C_<24>-, and C_<24>:_<1>-ceramide. This method was successfully applied to monitor the profiles of fatty acid lengths of ceramides in the stratum corneum. The profiles of fatty acid lengths of ceramides could be useful for the diagnosis of atopic dermatitis.

鞘脂代谢物如神经酰胺（角质层的主要脂质）已知在各种表皮功能中发挥关键作用。特应性皮炎患者通常表现出皮肤神经酰胺含量降低。我们开发了几种质谱方法（LC-MS/MS、MALDI-TOF 和 LC/MS-IT-TOF），用于快速、同步定量 C_<16> -、C_<18>-、C_<20>-、C_<22>-、C_<24>-和C_<24>:_<1>-神经酰胺。该方法成功应用于监测角质层中神经酰胺的脂肪酸长度分布。神经酰胺的脂肪酸长度特征可用于特应性皮炎的诊断。

项目成果

大腸癌細胞のスフィンゴ脂質代謝による抗癌剤感受性の制御機構

结直肠癌细胞鞘脂代谢调控抗癌药物敏感性的机制

• 发表时间: 2008
• 作者: 河野早紀;竹下未希;根本聡;中村光浩;伊藤善規;土屋照雄;村手隆;野澤義則;坂野喜子
• 通讯作者: 坂野喜子

Depletion of substance P, a mechanism for inhibition of mouse scratching behavior by tacrolimus

• DOI: 10.1016/j.ejphar.2009.09.043
• 发表时间: 2010-01-25
• 期刊: EUROPEAN JOURNAL OF PHARMACOLOGY
• 影响因子: 5
• 作者: Inagaki, Naoki;Shiraishi, Noriko;Nagai, Hiroichi
• 通讯作者: Nagai, Hiroichi

LCMS-IT-TOFによるスフィンゴシン脂質測定系の開発

使用LCMS-IT-TOF开发鞘氨醇脂质测量系统

• 发表时间: 2012
• 作者: 中材光浩;ら
• 通讯作者: ら

Analyses of benzodiazepines and their metabolites in various biological matrices by LC-MS(/MS)

• DOI: 10.1002/bmc.1598
• 发表时间: 2011-12-01
• 期刊: BIOMEDICAL CHROMATOGRAPHY
• 影响因子: 1.8
• 作者: Nakamura, Mitsuhiro

Characterization of Skin Inflammation Induced by Repeated Exposure of Toluene, Xylene, and Formaldehyde in Mice

• DOI: 10.1002/tox.20547
• 发表时间: 2011-06-01
• 期刊: ENVIRONMENTAL TOXICOLOGY
• 影响因子: 4.5
• 作者: Saito, Asaka;Tanaka, Hiroyuki;Nagai, Hiroichi
• 通讯作者: Nagai, Hiroichi