Analysis of myosin sliding on wide variety of actin-rails.

肌球蛋白在各种肌动蛋白轨道上滑动的分析。

• 批准号: 21590270
• 负责人: ISHIKAWA Ryoki
• 金额: $ 3万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590270/
• 关键词: アクチン; ミオシン; アクチン結合タンパク質; アクチン結合蛋白質

To examine whether or not brain-specific actin binding protein drebrin affects the activity of myosin-V, we observed the sliding of myosin-V on F-actin in the presence or absence of drebrin. We found that myosin-V slides on drebrin-decorated F-actin as fast as "naked" F-actin, whereas sliding distance and attachment time were smaller in the presence of drebrin than that in the absence of drebrin. Drebrin inhibits the binding of leading head to F-actin, but does not inhibit the release of ADP from trailing head. These results suggest that drebrin modulates the affinity of myosin-V to F-actin, thus inhibits the activity of myosin-V, and affects the motility of the central zone of nerve cell growth cones.

为了检查脑特异性肌动蛋白结合蛋白DREBRIN是否会影响肌球蛋白V的活性，我们观察到在存在或不存在DREBRIN的情况下，肌球蛋白V对F-肌动蛋白的滑动。我们发现，肌球蛋白V在Drebrin装饰的F-肌动蛋白上的滑动与“裸” F-肌动蛋白一样快，而在Drebrin的存在下，滑动距离和附着时间比没有Drebrin的时间小。 Drebrin抑制头部与F-肌动蛋白的结合，但不会抑制ADP从尾部释放。这些结果表明，Drebrin调节肌球蛋白V对F-肌动蛋白的亲和力，从而抑制肌球蛋白V的活性，并影响神经细胞生长锥的中央区域的运动性。

项目成果

The novel calmodulin-binding site of the smooth muscle myosin light chain kinase

平滑肌肌球蛋白轻链激酶的新钙调蛋白结合位点

• 发表时间: 2010
• 作者: Nakamura;A.;Matsumoto;A.;Xie;C.;Yoshiyama;S.;Ishikawa;R.;Kohama;K.
• 通讯作者: K.

Down-Regulation of Myosin Light Chain Kinase Expression in Vascular Smooth Muscle Cells Accelerates Cell Proliferation: Requirement of Its Actin-binding Domain for Reversion to Normal Rates

血管平滑肌细胞中肌球蛋白轻链激酶表达的下调加速细胞增殖：需要其肌动蛋白结合域才能恢复正常速率

• DOI: 10.1254/jphs.11213sc
• 发表时间: 2012
• 期刊: Journal of Pharmacological Sciences
• 影响因子: 3.5
• 作者: Wang HH;Nakamura A;Yoshiyama S;Ishikawa R;Cai N;Ye LH;Takano-Ohmuro H;Kohama K
• 通讯作者: Kohama K

Modulation of the mechano-chemical properties of myosin V by drebrin-E

• DOI: 10.1016/j.bbrc.2010.08.120
• 发表时间: 2010-10-01
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kubota, Hiroaki;Ishikawa, Ryoki;Ishiwata, Shin'ichi
• 通讯作者: Ishiwata, Shin'ichi

Utilization of myosin and actin bundles for the transport of molecular cargo

利用肌球蛋白和肌动蛋白束运输分子货物

• 发表时间: 2010
• 期刊: Small
• 影响因子: 13.3
• 作者: Takatsuki H;Rice KM;Asano S;Day BS;Hino M;Oiwa K;Ishikawa R;Hiratsuka Y;Uyeda TQ;Kohama K;Blough ER
• 通讯作者: Blough ER

Stimulatory effects of arachidonic acid on myosin ATPase activity and contraction of smooth muscle via myosin motor domain

花生四烯酸通过肌球蛋白运动域对肌球蛋白 ATP 酶活性和平滑肌收缩的刺激作用

• 发表时间: 2010
• 期刊: Am.J.Physiol.Heart Circ.Physiol. 298
• 作者: Katayama;T.;Watanabe;M.;Tanaka;H.;Hino;M.;Miyakawa;T.;Ohki;T.;Ye;L-H.;Xie;C.;Yoshiyama;S.;Nakamura;A.;Ishikawa;R.;Tanokura;M.;Oiwa;K.;Kohama;K.
• 通讯作者: K.