Regulation of ciliary beat frequency by sex hormones in ciliary cells of Fallopian tube.

输卵管睫状细胞中性激素对纤毛跳动频率的调节。

• 批准号: 21590243
• 负责人: NAKAHARI Takashi
• 金额: $ 3万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590243/
• 关键词: Fallopian tube; cilia; ciliary beat frequency; estrogen; progesterone; egg transport; ovarian cycle; oviduct epithelia; oviduct cilia; Egg transport; egg trans; infertilization; Ciliary beat frequency

The ciliary beat frequency(CBF) of guinea pig fimbria during the ovarian cycle was measured by video microscopy using a high-speed camera(500Hz). A moderate increase in estrogen concentration, [βE_2], maintains a high CBF(15.5Hz) during the ovulation and then a further increase in [βE_2] decreases CBF(13.5 Hz) in the ovulatory phase. In the early and late luteal phase, low [βE_2] and progesterone concentration, [PRG], increase CBF to 17Hz and 15Hz, respectively, and in the middle luteal phase, a high [PRG] decreases CBF(～ 12Hz). Thus, the CBF of the fimbria was controlled by signals via βE_2 and PRG receptors in guinea pigs. Moreover both βE_2 and PRG antagonized each other in CBf regulation, that is βE_2 inhibits PRG actions and contrary, PRG inhibits βE_2 actions.

在卵巢周期期间，豚鼠纤维的纤毛搏动频率（CBF）使用高速摄像头（500Hz）测量视频显微镜。雌激素浓度的现代增加[βE_2]在排卵过程中保持高CBF（15.5Hz），然后在排卵阶段进一步增加[βe_2]。在黄体早期和晚期，低[βe_2]和孕酮浓度分别将CBF增加到17Hz和17Hz和15Hz，在中间黄体期间，高[PRG]降低了CBF（〜12Hz）。那就是脂肪症的CBF通过豚鼠中的βe_2和PRG受体来控制。此外，βE_2和PRG在CBF调节中彼此拮抗，即βE_2抑制PRG的作用并对比，PRG抑制了βe_2的作用。

项目成果

The regulation of ciliary beat frequency by ovarian steroids in the guinea pig Fallopean tube : interactions between oestradiol and progesterone

豚鼠输卵管中卵巢类固醇对纤毛跳动频率的调节：雌二醇和黄体酮之间的相互作用

• 发表时间: 2011
• 期刊: Biomed Res
• 作者: Nakahari T;Nishimura A;Shimamoto C;Sakai A;Kuwabara H;Nakano T;Tanaka S;Kohda Y;Matsumura H;Mori H.
• 通讯作者: Mori H.

HCO_3-dependent transient acidification induced by ionomycin in rat submandibular acinar cells

离子霉素诱导大鼠颌下腺泡细胞HCO_3依赖性瞬时酸化

• 发表时间: 2010
• 期刊: Journal of Physiological Sciences
• 影响因子: 2.3
• 作者: Yoshida H.;et al.
• 通讯作者: et al.

生体防御バリアーとしての上皮機能の活性化因子の研究:卵管線毛運動の卵巣周期による変化.エストロゲンとプロゲステロンによるコントロール

作为生物防御屏障的上皮功能激活剂的研究：输卵管纤毛运动的变化取决于雌激素和孕激素的控制。

• 发表时间: 2009
• 期刊: 大阪医科大学雑誌
• 作者: 西村歩;中張隆司;島本史夫;中張隆司
• 通讯作者: 中張隆司

PDE1 modulation of ciliary beat frequency, not ciliary bend angle, in β2-agonist, procaterol stimulated mouse bronchiolar ciliary cells

在 β2 激动剂、丙卡特罗刺激的小鼠细支气管纤毛细胞中，PDE1 调节纤毛跳动频率，而不是纤毛弯曲角度

• 发表时间: 2012
• 作者: Suzuki T;et al;Nakahari T
• 通讯作者: Nakahari T

Peroxisome proliferation activation receptor α modulation of Ca2+-regulated exocytosis via arachidonic acid in guinea-pig antral mucous cells

• DOI: 10.1113/expphysiol.2010.053603
• 发表时间: 2010-08-01
• 期刊: EXPERIMENTAL PHYSIOLOGY
• 影响因子: 2.7
• 作者: Sawabe, Yukinori;Shimamoto, Chikao;Nakahari, Takashi
• 通讯作者: Nakahari, Takashi