Molecular and morphological analysis of RNA helicase that shows an unique expression in the nervous system by utilizing genetically modified animals

利用转基因动物对 RNA 解旋酶进行分子和形态学分析，该酶在神经系统中显示出独特的表达

• 批准号: 21590198
• 负责人: TSURUO Yoshihiro
• 金额: $ 3万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590198/
• 关键词: RNAヘリカーゼ; 神経系; オリゴデンドロサイト; 遺伝子改変動物; 分子形態学

The monoclonal antibody(4F2) that specifically identifies oligodendrocytes was generated, and the molecule recognized by this antibody proved to be Ddx54, a member of RNA helicase. In the transgenic animals with forced expression of Ddx54, myelination appeared to be accelerated. The 4F2-positive cells were observed in the rat central nervous system from early embryonal to adult stages, and they were specifically expressed in oligodendrocytes using primary cultures of neural tissues. Ddx54 bound to 4 types of MBP isoforms, and especially the 21. 5 kDa isoform of MBP was transported into the nucleus, suggesting its involvement in the signal transduction during the process of myelination in oligodendrocytes.

产生了特异性识别少突胶质细胞的单克隆抗体（4F2），该抗体识别的分子被证明是RNA解旋酶成员Ddx54。在强制表达 Ddx54 的转基因动物中，髓鞘形成似乎加速。在大鼠中枢神经系统中从胚胎早期到成年阶段均观察到4F2阳性细胞，并且利用神经组织原代培养物在少突胶质细胞中特异性表达。 Ddx54 与 4 种 MBP 亚型结合，特别是 21. 5 kDa 的 MBP 亚型被转运至细胞核，表明其参与少突胶质细胞髓鞘形成过程中的信号转导。

项目成果

オリゴデンドロサイトに特異的モノクローナル抗体(4F2)による新規分子マーカーの同定および機能解析

使用少突胶质细胞特异性单克隆抗体（4F2）鉴定新型分子标记并进行功能分析

• 发表时间: 2012
• 作者: 山本悠太;鶴尾吉宏;志波歩美;伊藤隆雄;上山敬司;植木俊之;山本雅浩;阿相皓晃
• 通讯作者: 阿相皓晃