Immunologic functional analysis of staphylococcal enterotoxin on canine atopic dermatitis

葡萄球菌肠毒素对犬特应性皮炎的免疫功能分析

• 批准号: 21580390
• 负责人: FUKADA Tsuneo
• 金额: $ 3.24万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580390/
• 关键词: アトピー性皮膚炎; 犬; エンテロトキシン; ブドウ球菌; rSEC_<canine>; S.psuedintermedius; ケラチノサイト; ケモカイン; 膿皮症; S.pseudintermedius; staphylococcal enterotoxin; SEC_<canine>; リンパ球刺激能; multiplex-PCR; SECcanine

To study of immunologic functional analysis of staphylococcal enterotoxin on canine atopic dermatitis, Staphylococcus from canine pyoderma were isolated and identified. Staphylococcus pseudintermedius(SP) is one of the major organism and many SP are methicillin-resistant strains. These staphylococci produce staphylococcal enterotoxins(SE-s). Recombinant SEC-canine protein(rSEC-canine) using expression vector system by Escherichia coli was purified and its rSEC-canine has in vitro canine lymphocyte-stimulating active. Using a cell line of canine epidermal keratinocyted, mRNA transcription of TNF-α, CCL17 and CCL28 was significantly augmented by rSEC-canine stimulation. SEC-canine is an important inducing factor for the production of not only TNF-α, but also CCL17 and CCL28, which are important chemokines in the pathogenesis of canine AD.

在犬类特应性皮炎上鉴定出葡萄球菌性皮炎的葡萄球菌肠毒素的免疫学分析。犬纯化的大肠杆菌的RSEC-甲烷在体外具有刺激性的刺激性刺激性。犬是非生产的重要诱导因子，但也是CCL17和CCL28，它们是犬AD发病机理中重要的趋化因子。

项目成果

Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi Subsp

假中间葡萄球菌和施莱菲葡萄球菌亚种的抗菌药物敏感性和甲氧西林耐药性

• 发表时间: 2010
• 期刊: coagulans Isolated from Dogs with Pyoderma in Japan. J Vet Med Sci
• 作者: Kawakami T;Shibata S;Murayama N;Nagata M;Nishifuji K;Iwasaki T;Fukada T
• 通讯作者: Fukada T

膿皮症由来Staphylococcus pseudintermediusにおける多剤耐性およびメチシリン耐性状況

脓皮病来源的伪中间葡萄球菌的多药耐药性和甲氧西林耐药状况

• 发表时间: 2010
• 作者: 川上哲司;柴田早苗;林玲子;深町綾;村山信雄;永田雅彦;西藤公司;岩崎利郎;深田恒夫
• 通讯作者: 深田恒夫

Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus pseuthntermedius and Staphylococcus schleikri Subsp. coagulans Isolated from Dogs with Pyoderma in Japan.

假中葡萄球菌和施莱克葡萄球菌亚种的抗菌药物敏感性和甲氧西林耐药性。

• 发表时间: 2010
• 期刊: The Journal of Veterinary Medical Science
• 作者: Kawakami;T.;et. al.
• 通讯作者: et. al.