Effects of VEGF and its receptors on differentiation and proliferation of mammary gland and new born digestive tract.

VEGF及其受体对乳腺及新生儿消化道分化和增殖的影响。

• 批准号: 21580366
• 负责人: MATSUMOTO Mitsuharu
• 金额: $ 3万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580366/
• 关键词: VEGF; VEGF受容体; NOS; 免疫組織化学; 乳腺; 新生仔消化管; 分化増殖; マウス; VEGFR1; VEGFR2; 十二指腸; ネコ; 乳汁; 消化管

The effects of VEGF and its receptors on the differentiation and proliferation of mammary gland and new born digestive tract were investigated by immunohistochemistry, histometry and biochemistry. In mouse mammary gland during reproductive cycle, VEGF and NOS(eNOS and bNOS) which generate a free radical gas, NO, expressed strongly in late pregnancy and early lactation. VEGFR-2, one of VEGF receptors, was localized to blood vessel endothelia. In feline mammary tumors, the expressions of VEGF and iNOS were positively correlated with tumor grades. On the other hands, in mouse digestive tract, VEGF was localized to absorptive and glandular epithelium, VEGFR-1 to striated border, VEGFR-2 to blood vessel epithelium. The expressions of these proteins became strongly in advance with dating. After administration of VEGF in the digestive tract, immunoreaction of VEGFR-1 was significantly decreased. Thus, it is suggested that these proteins mentioned above were intimately correlated with the differentiation and proliferation, and angiogenesis in mammary gland and new born digestive tract.

采用免疫组化、组织学和生物化学方法研究VEGF及其受体对乳腺和新生儿消化道分化和增殖的影响。在生殖周期的小鼠乳腺中，VEGF和NOS（eNOS和bNOS）产生自由基气体NO，在妊娠晚期和哺乳早期强烈表达。 VEGFR-2 是 VEGF 受体之一，定位于血管内皮细胞。在猫乳腺肿瘤中，VEGF和iNOS的表达与肿瘤分级呈正相关。另一方面，在小鼠消化道中，VEGF定位于吸收性上皮和腺上皮，VEGFR-1定位于横纹缘，VEGFR-2定位于血管上皮。这些蛋白质的表达随着年代测定的提前而变得强烈。在消化道中施用VEGF后，VEGFR-1的免疫反应显着降低。由此表明，上述这些蛋白与乳腺和新生消化道的分化、增殖、血管生成密切相关。

项目成果

乳汁中のVEGFと新生仔消化管におけるVEGF受容体の局在

牛奶中 VEGF 和新生儿胃肠道中 VEGF 受体的定位

• 发表时间: 2009
• 作者: 石田理奈;松元光春;藤田あゆみ;叶内宏明;岡達三
• 通讯作者: 岡達三

Change in VEGF Expression in Mouse Mammary Gland during Reproductive Cycle

• DOI: 10.1292/jvms.10-0086
• 发表时间: 2010-09-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Islam, Mohammad Saiful;Matsumoto, Mitsuharu;Kanouchi, Hiroaki
• 通讯作者: Kanouchi, Hiroaki

マウス乳腺におけるグレリンと細胞動態に関する免疫組織化学的解析

小鼠乳腺生长素释放肽和细胞动力学的免疫组织化学分析

• 发表时间: 2010
• 作者: 阿部和己;辻尾祐志;松元光春
• 通讯作者: 松元光春

イヌの正常乳腺組織におけるレクチン組織化学的研究

狗正常乳腺组织的凝集素组织化学研究

• 发表时间: 2009
• 作者: 貝塚左希子;松元光春;三好宣彰;安田宣紘
• 通讯作者: 安田宣紘

Expression and localization of VEGFR-2 in mouse mammary gland during reproductive cycle

VEGFR-2在小鼠生殖周期乳腺中的表达和定位

• 发表时间: 2010
• 作者: Islam MS;松元光春;辻尾祐志
• 通讯作者: 辻尾祐志