Investigation of Basal Ganglia Function and Intrinsic Dopamine Release by Positron Emission Tomography

正电子发射断层扫描研究基底神经节功能和内在多巴胺释放

• 批准号: 12670635
• 负责人: ISHII Kenji
• 金额: $ 2.05万
• 依托单位: Tokyo Metropolitan Institute of Gerontology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670635/
• 关键词: PET; dopamine; raclopride; basal ganglia; voluntary movement; learning; human; brain; ポジトロンCT; ドーパミン放出; 書字; 脳賦活検査; ドーパミン受容体

To investigate the role of nigrostriatal dopamine system in performing a skilled complex motor task without leaning such as writing, we measured endogenous dopamine release in human brain with ^<13>C-raclopride (RAC) and PET.We studied six right-handed healthy volunteers (ages 22.0±1.7 years). After an intravenous injection of RAC, a 60 minites dynamic scan was performed. Each subject participated in RAC-PET twice on the same day (scan interval 145.0±35.2 minutes) under two different conditions ; during hand writing and at rest in balanced order. In the writing condition, the subjects was asked to dictate sentences as quick as possible on a computer-controlled writing pad with pen tool using their right hand. In resting condition, the subject was asked to watch the display without any movement. Endgeneous dopamine release was estimated indirectly based on the occupancy model, from the difference of the binding potential (BP) of PAC in two different conditions. The same subjects also participated in H_<2>^<15>O-PET with the same paradigm to evaluate the regional cerebral activation with the task in terms of regional cerebral blood flow change.The left striatum was activated significantly with H_<2>^<15>5O-PET study with writing task. In the same region, the binding potentials of RAC was significantly reduced by dictation task, thus, the intrinsic dopamine release was considered to be accelerated by the task. The activated region located in the ventro-caudal portion of the putamen which we previosly reported to be corresponding to hand area in the sometotopic organization in the basal ganglia. This result demonstrated the ability of indirect measurement method of intrinsic dopamine release by RAC-PET in physiological condition. Our results are the first report of activation of dopaminergic neurons by a skilled voluntary monements.

为了研究黑质纹状体多巴胺系统在无需学习的情况下执行熟练的复杂运动任务（例如书写）中的作用，我们用^ 13 C-雷氯必利（RAC）和PET测量了人脑中内源性多巴胺的释放。我们研究了六名右手健康人志愿者（年龄22.0±1.7岁）静脉注射RAC后，每个受试者在同一天进行两次60分钟的动态扫描。 （扫描间隔145.0±35.2分钟）在两种不同的条件下；在手写和休息时以平衡顺序书写的条件下，受试者被要求使用笔工具在计算机控制的书写板上尽可能快地听写句子。在静止状态下，受试者被要求在没有任何运动的情况下观看显示器，根据占用模型，根据两种不同条件下 PAC 的结合电位 (BP) 的差异来间接估计内源性多巴胺释放。相同的受试者还参加了具有相同范式的H_<2>^<15>O-PET，以评估局部脑激活，任务是局部脑血流变化。H_<2>^ 左侧纹状体显着激活15O-PET研究中，在同一区域，听写任务显着降低了RAC的结合电位，因此，认为该任务加速了内在的多巴胺释放。我们之前报道的壳核的腹尾部分对应于基底神经节中的同位组织中的手部区域。这一结果证明了在生理条件下通过 RAC-PET 间接测量内在多巴胺释放的方法的能力。首次报道熟练的自愿分子激活多巴胺能神经元。

项目成果

Nariai, T., Ohno, K., Ohta, Y., Hirakawa, K., Ishii, K. and Senda, M.: "Discordance between cerebral oxygen and glucose metabolism, and hemodynamics in a mitochondrial encephalomyopathy, lactic acidosis, and strokelike episode.patient"J Neuroimag,. 11(3).

Nariai, T.、Ohno, K.、Ohta, Y.、Hirakawa, K.、Ishii, K. 和 Senda, M.：“线粒体脑肌病、乳酸性酸中毒和脑氧代谢和葡萄糖代谢与血流动力学之间的不一致

石井賢二: "Parkinson病の機能画像"Clinical Neurosci. 19. 32-34 (2001)

Kenji Ishii：“帕金森病的功能成像”《临床神经科学》19. 32-34 (2001)。

Maillard, M. et al.: "Mapping the Basal Ganglia -fMRI evidence for somatotopic representation of face, hand, and foot"Neurology. 55. 377-383 (2000)

Maillard, M. 等人：“绘制基底神经节图 - 面部、手部和足部体位表征的 fMRI 证据”神经病学。

Mishina, M. et al.: "Intrasubject correlation between static scan and distribution volume images for [C] flumazenil PET"Ann Nuol Med. 14. 193-198 (2000)

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Suzuki, Y., Kiyosawa, M., Mochizuki, M., Ishii, K. and Senda, M.: "Cortical blindness following aortic arch surgery"Jpn J Ophthalmol. 45. 547-549 (2001)

Suzuki, Y.、Kiyosawa, M.、Mochizuki, M.、Ishii, K. 和 Senda, M.：“主动脉弓手术后皮质失明”Jpn J Ophamol。