The basic and clinical study of the relevance of periodontal disease and metabolic syndrome.

牙周病与代谢综合征相关性的基础与临床研究。

• 批准号: 20890143
• 负责人: YAMASHITA Akiko
• 金额: $ 2.11万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20890143/
• 关键词: アディポサイトカイン; 歯周病; メタボリックシンドローム

We analyzed genes differentially expressed in adipocytes when co-cultured with macrophages in the presence of a ligand TLR-4, bacterial lipopolysaccharide (LPS). RAW264.7, a murine macrophage cell line and differentiated 3T3-L1 adipocytes were co-cultured using a transwell system. Genes differentially expressed in adipocytes were analyzed by the DNA microarray method. Co-culture of macrophages and adipocytes with a low LPS concentration markedly upregulated gene expressions associated with inflammation and/or angiogenesis, such as those of IL-6, MCP-1, RANTES and CXCL1/KC, in adipocytes.

我们分析了在配体 TLR-4、细菌脂多糖 (LPS) 存在的情况下与巨噬细胞共培养时脂肪细胞中差异表达的基因。使用 Transwell 系统共培养小鼠巨噬细胞系 RAW264.7 和分化的 3T3-L1 脂肪细胞。通过DNA微阵列方法分析脂肪细胞中差异表达的基因。巨噬细胞和脂肪细胞与低 LPS 浓度的共培养显着上调脂肪细胞中与炎症和/或血管生成相关的基因表达，例如 IL-6、MCP-1、RANTES 和 CXCL1/KC。

项目成果

DNA microarray analyses of genes expressed differentially in 3T3-L1 adipocytes co-cultured with murine macrophage cell line RAW264.7 in the presence of the toll-like receptor 4 ligand bacterial endotoxin

• DOI: 10.1038/ijo.2008.153
• 发表时间: 2008-11-01
• 期刊: INTERNATIONAL JOURNAL OF OBESITY
• 影响因子: 4.9
• 作者: Yamashita, A.;Soga, Y.;Nishimura, F.
• 通讯作者: Nishimura, F.

DNA microarray analyses of LPS-stimulated adipocytes co- cultured with macrophages

LPS 刺激的脂肪细胞与巨噬细胞共培养的 DNA 微阵列分析

• 发表时间: 2009
• 作者: Akiko Yamashita;et al
• 通讯作者: et al

Chronic low-grade inflammation accelerating aging

慢性低度炎症加速衰老

• 发表时间: 2009
• 期刊: Inflammation and Regeneration 29(3)
• 作者: Fusanori Nishimura;Yoshihiko Soga;Akiko Yamashita;Periodontal disease
• 通讯作者: Periodontal disease

脂肪細胞-マクロファージ相互作用から捉えた, 肥満症における炎症の増幅機序の解明

基于脂肪细胞-巨噬细胞相互作用阐明肥胖炎症放大机制

• 发表时间: 2008
• 作者: 山下明子;他
• 通讯作者: 他

Cytokine Profile Produced from LPS- stimulated Macrophage- Adipocyte Co-cultures

LPS 刺激的巨噬细胞-脂肪细胞共培养物产生的细胞因子谱

• 发表时间: 2008
• 作者: Akiko Yamashita;et al
• 通讯作者: et al