Morphological study of the diacylglycerol kinase family

二酰甘油激酶家族的形态学研究

• 批准号: 20700312
• 负责人: HOZUMI Yasukazu
• 金额: $ 2.75万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20700312/
• 关键词: ジアシルグリセロールキナーゼ; 免疫組織化学染色; 特異抗体; 共焦点レーザー顕微鏡; 蛍光多重染色法; ポストシナプス; 免疫電子顕微鏡法; 細胞膜

DGKβ is expressed not only in projection neurons but also in interneurons and is concentrated at perisynaptic sites of asymmetrical synapses. Overexpression of wild-type DGKβ promotes dendrite outgrowth at 7 d in vitro (DIV) and spine maturation at 14 DIV in transfected hippocampal neurons, although its kinase-dead mutant has no effect. In addition, we show that DGKβ and dopamine D2 receptor are co-expressed in the intermediate lobe and localize to plasma membrane side by side. These findings will substantiate and further extend our understanding of the molecular-anatomical pathway of the phosphoinositide signaling and functional roles of DGK in the pituitary intermediate lobe.

DGKβ不仅在投影神经元中表达，而且集中在不对称突触的周围对称部位。野生型DGKβ的过表达在体外（DIV）7天（DIV）和脊柱成熟在转染的海马神经元中的14 div促进了树突生长，尽管其激酶死去的突变体没有作用。此外，我们表明DGKβ和多巴胺D2受体在中间叶中共表达，并将其本地化至质膜。这些发现将证实并进一步扩展了我们对DGK在居住的中间叶中DGK的磷酸肌醇信号传导和功能作用的分子原子学途径的理解。

项目成果

Diacylglycerol kinase beta promotes dendritic outgrowth and spine maturation in developing hippocampal neurons.

• DOI: 10.1186/1471-2202-10-99
• 发表时间: 2009-08-19
• 期刊: BMC neuroscience
• 影响因子: 2.4
• 作者: Hozumi Y;Watanabe M;Otani K;Goto K
• 通讯作者: Goto K

Diacylglycerol kinase zeta is associated with chromatin, but dissociates from condensed chromatin during mitotic phase in NIH3T3 cells

二酰甘油激酶 zeta 与染色质相关，但在 NIH3T3 细胞有丝分裂期与浓缩染色质分离

• 发表时间: 2008
• 期刊: J Cell Biochem 105
• 作者: Hasegawa H;et.al.
• 通讯作者: et.al.

Signaling cascade of diacylglycerol kinase in the pituitary intermediate lobe: dopamine D2 receptor/ phospholipaseCbeta4/diacylglycerol kinase beta / protein kinase Calpha.

垂体中叶二酰甘油激酶的信号级联：多巴胺 D2 受体/磷脂酶 Cbeta4/二酰甘油激酶 beta/蛋白激酶 Calpha。

• 发表时间: 2010
• 期刊: J Histochem Cytochem 58
• 作者: Hozumi Y;Watanabe M;Goto K.
• 通讯作者: Goto K.

Diacylglycerol kinase β promotes dendritic outgrowth and spine maturation in developing hippocampal neurons

二酰甘油激酶β促进发育中海马神经元的树突生长和脊柱成熟

• 发表时间: 2009
• 作者: Noriaki Ohkawa;Yoshito Saitoh;Eri Tokunaga;Toshio Kitamura;Kaoru Inokuchi;大川宜昭;Hozumi Y.;大川宜昭;Hozumi Y.
• 通讯作者: Hozumi Y.

Diacylglycerol kinase β promotes dendritic outgrowth and spine maturation in developing hippocampal neurons.

二酰甘油激酶β促进发育中的海马神经元的树突生长和脊柱成熟。

• 发表时间: 2009
• 作者: Noriaki Ohkawa;Yoshito Saitoh;Eri Tokunaga;Toshio Kitamura;Kaoru Inokuchi;大川宜昭;Hozumi Y.
• 通讯作者: Hozumi Y.