Tissue engineering by using electrochemical approaches

使用电化学方法进行组织工程

• 批准号: 20686056
• 负责人: FUKUDA Junji
• 金额: $ 15.64万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20686056/
• 关键词: 医用化学工学; 再生医療; 自己組織化単分子膜; 細胞組織工学; バイオ関連機器; 細胞チップ; マイクロアレイ

This study demonstrated that cells were detached within 5 min via electrical desorption of alkane thiol self-assembled monolayers. This is the most rapid cell detachment approach compared to other previous approaches. This electrical approach could also be used to harvest cell sheets of cardiomyocytes and fibroblasts, and hepatocyte spheroids. Furthermore, this was applicable to fabricate vascular-like structures, by transferring cells to the internal surface of microchannels in hydrogel. This approach can be a fundamental tool for engineering tissues with vascular networks.

这项研究表明，通过烷硫醇自组装单层的电解吸，细胞在 5 分钟内分离。与之前的其他方法相比，这是最快速的细胞分离方法。这种电方法还可用于收获心肌细胞和成纤维细胞以及肝细胞球体的细胞片。此外，这适用于通过将细胞转移到水凝胶中微通道的内表面来制造血管样结构。这种方法可以成为构建具有血管网络的组织的基本工具。

项目成果

Electrochemical desorption of self-assembled monolayers for engineering cellular tissues

• DOI: 10.1016/j.biomaterials.2009.03.045
• 发表时间: 2009-07-01
• 期刊: BIOMATERIALS
• 影响因子: 14
• 作者: Inaba, Rina;Khademhosseini, Ali;Fukuda, Junji
• 通讯作者: Fukuda, Junji

Engineering Two- and Three-dimensional Cellular Aggregates by Electrochemical Reaction

通过电化学反应工程化二维和三维细胞聚集体

• 发表时间: 2008
• 作者: Rina Inaba;Hiroaki Suzuki;Junji Fukuda
• 通讯作者: Junji Fukuda

Rapid Diagnostic Device for Subclinical Mastitis Based on Electrochemical Detection of Superoxide Produced from Neutrophils in Fresh Milk

基于电化学检测鲜奶中中性粒细胞产生的超氧化物的亚临床乳腺炎快速诊断装置

• 发表时间: 2011
• 期刊: IEEJ Transactions on Sensors and Micromachines
• 作者: K.Okada;J.Fukuda;H.Suzuki
• 通讯作者: H.Suzuki

Gold-black micropillar electrodes for microfluidic ELISA of bone metabolic markers

• DOI: 10.1039/c0an00619j
• 发表时间: 2011-01-01
• 期刊: ANALYST
• 影响因子: 4.2
• 作者: Numthuam, Sonthaya;Ginoza, Takahiro;Fukuda, Junji
• 通讯作者: Fukuda, Junji

Fabrication of Patterned Cell Co-Cultures on Albumin-Based Substrate

在白蛋白基质上构建图案化细胞共培养物

• 发表时间: 2010
• 期刊: Acta Biomaterialia 6
• 作者: H.Yamazoe;T.Okuyama;H.Suzuki;J.Fukuda
• 通讯作者: J.Fukuda