Identification for TGF-beta signaling during palatal development

腭发育过程中 TGF-β 信号传导的鉴定

• 批准号: 20592415
• 负责人: NAKAJIMA Akira
• 金额: $ 3万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592415/
• 关键词: TGF-beta; 口蓋裂; 二次口蓋; 分子生物学; 成長・発育

The molecular mechanisms regulating palatogenesis remain incompletely characterized but are most likely to be involved in the molecular etiology for cleft palate. The objective was to investigate TGF-β3 signaling when both TGF-β type II and III receptors (TβR-II/III) were knocked down an NIH3T3 cell line and palatal organ culture in vitro. Treatment with siTβR-II/III reduced the expression of the target genes. The treated palates were partially fused at E13+72h when TβR-II/III were knocked down,although all control palate were completely fused. The downstream genes was decreased in the siTβR-II/III treated NIH3T3 cells and palatal shelves. The present study demonstrated that knocking down both TβR-II and III could affect the downstream signaling pathway of TGF-β by reducing the levels of phospho-Smad2. The siTβR-II/III treatment resulted in persistent MEE cell proliferation, which has been shown to be linked to a failure to complete palatal fusion events.

调节腭裂的分子机制尚未完全确定，但最有可能涉及腭裂的分子病因学。目的是研究当 TGF-β II 型和 III 型受体 (TβR-II/III) 均同时存在时 TGF-β3 信号传导。体外敲低 NIH3T3 细胞系和腭器官培养物，用 siTβR-II/III 处理降低了目标基因的表达，在 E13+72h 时，处理后的腭部分融合。当 TβR-II/III 被敲低时，尽管所有对照腭都完全融合，但在 siTβR-II/III 处理的 NIH3T3 细胞和腭架中下游基因有所减少。 siTβR-II/III 治疗可导致 MEE 细胞持续增殖，这与以下因素有关：未能完成腭融合事件。

项目成果

IL-17A stimulates the expression of inflammatory cytokines via celecoxib-blocked prostaglandin in MC3T3-E1 cells

IL-17A 通过塞来昔布阻断的前列腺素刺激 MC3T3-E1 细胞中炎症细胞因子的表达

• 发表时间: 2010
• 期刊: Arch Oral Biol 55
• 作者: Zhang F;Koyama Y;Sanuki R;Mitsui N;Suzuki N;Kimura A;Nakajima A;Shimizu N;Maeno M.
• 通讯作者: Maeno M.

TGF-beta type II and III receptors signaling for fate of MEE in palatogenesis

TGF-β II 型和 III 型受体信号传导 MEE 在腭发育中的命运

• 发表时间: 2010
• 作者: Nakajiama A;Ito Y;Mitsui N;Maeno K;Iwata K;Shuler CF;Shimizu N
• 通讯作者: Shimizu N

The functional role of TβR-II and III receptors during palatal fusion

TβR-II 和 III 受体在腭融合过程中的功能作用

• 发表时间: 2011
• 作者: Nakajiama A;Ito Y;Tanaka E;Iwata K;Maeno M;Shimizu N;Shuler CF
• 通讯作者: Shuler CF

TGF-β type III receptorにおけるsignaling pathwayの解明

TGF-β III 型受体信号通路的阐明

• 发表时间: 2008
• 作者: 中嶋昭;他;松澤光洋;中嶋昭,浅野正岳,三井教祐,Shuler CF,清水典佳
• 通讯作者: 中嶋昭,浅野正岳,三井教祐,Shuler CF,清水典佳

二次口蓋融合におけるTGF-β type II/III receptor signaling pathwayの解明

阐明二次腭融合中 TGF-β II/III 型受体信号通路

• 发表时间: 2010
• 作者: 山口三菜;進士久明;木本茂成;倉田茂昭;大川浩作;山本浩之;中嶋昭,三井教裕,前野正夫,Charles F Shuler,清水典佳
• 通讯作者: 中嶋昭,三井教裕,前野正夫,Charles F Shuler,清水典佳