Development of new and safer denture cleanser with Erythritol

开发新型更安全的赤藓糖醇假牙清洁剂

基本信息

批准号：
20592271
负责人：
KASHIWABARA Toshiya
金额：
$ 2.75万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

The purpose of the present study was to develop a new and safer denture cleanser with "Erythritol", one of sugar alcohols for preventing dental caries, periodontal disease, stomatitis and aspiration pneumonia through dental and denture plaque control.In vitro experiment, candidal biofilm formation and assessment of sterilized effect were established. An in vitro Candida albicans biofilm was formed on a plastic coverslip coated with type I collagen following two methods and were compared : 1) yeast nitrogen base (YNB) /100 mM glucose medium supplemented with 2.5 mM N-acetylglucosamine (GlcNAc) ; and 2) BHI medium. The former method was employed in this experiment. The quantitative CFU counting in SEM images was employed as the assessment of sterilized effect. The enhancement of the fungicidal effect was evaluated using microbial assay after treating the biofilm with the test solutions (sugar alcohols : erythritol, xylitol, and sorbitol, each containing BTC solution). No fungicidal effect was observed with sugar alcohols without BTC. Thefungicidal effect of erythritol-containing BTC increased with the erythritol concentration. Further, the level of enhancement of erythritol was the highest in three sugar alcohols. Sugar alcohols, especially erythritol, enhanced the fungicidal effect of sterilized agents toward in vitro candidal biofilms. In vivo experiment, the sterilized effect of the solution with and without erythritol on the sterilization of periodontal pocket was evaluated by Randomized Control Trials and PCR test. The sterilized effect of the solution with erythritol was somewhat higher.The sterilized solution with sugar alcohols, especially erythritol would be a safe and effective denture cleanser.

本研究的目的是用“促红节醇”开发一种新的，更安全的义齿清洁剂，这是一种用于预防龋齿，牙周疾病，牙周疾病，口腔炎和抽吸肺炎通过牙齿和牙齿牙菌斑控制。遵循两种方法，在涂有I型胶原蛋白的塑料盖玻片上形成了体外白色念珠菌生物膜，并进行了比较：1）酵母氮碱（YNB） /100 mM葡萄糖培养基，该葡萄糖培养基添加了2.5 mM N-乙酰基葡萄糖胺（GlCNAC）； 2）BHI培养基。前一种方法用于该实验。 SEM图像中的定量CFU计数被用作对灭菌作用的评估。使用测试溶液（糖醇：乙醇醇，木糖醇和山梨糖醇）处理生物膜后，使用微生物测定法评估了杀真菌效应的增强。没有BTC的糖醇未观察到杀菌作用。含红氨酸的BTC的f肺作用随促红醇浓度增加。此外，促红醇的增强水平是三种糖中的最高水平。糖醇，尤其是赤霉菌，增强了灭菌剂对体外候选生物膜的杀菌作用。在体内实验中，通过随机对照试验和PCR测试评估了带有和没有促红细球的溶液对牙周口袋灭菌的灭菌作用。用赤券醇的溶液对溶液的灭菌作用较高。用糖醇（尤其是赤藻醇）灭菌的溶液将是一种安全有效的牙齿清洁剂。