Mesenchymal stem cells infected with modified adenoviruses as carrier cells that target human gastrointestinal tumors

感染修饰腺病毒的间充质干细胞作为靶向人类胃肠道肿瘤的载体细胞

• 批准号: 20591585
• 负责人: TAGAWA Masatoshi
• 金额: $ 3万
• 依托单位: Chiba Cancer Center (Research Institute)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591585/
• 关键词: 食道がん; がん治療; 遺伝子; アデノウイルス; キャリアー細胞; 遺伝子治療; 制限増殖型ウイルス; 間葉系幹細胞; アポトーシス; 細胞傷害活性; 遺伝子医薬; コクサキーアデノウイルス受容体; CD46分子; p53遺伝子; 初期応答遺伝子; ウイルス受容体

Majority of human solid tumors, when clinically in an advanced stage, is resistant to most of conventional therapies and consequently improved prognosis with a novel strategy is a crucial target in clinical settings. We developed type 5 adenoviruses which replicated preferentially within tumors and then examined the anti-tumor effects. We also modified the adenoviruses of which the receptor-binding site was replaced with that of type 35 adenoviruses, which subsequently increased the infectivity to human tumors due to a high receptor expression in the tumors. We examined a possible advantage to use carrier cells which were infected with such adenoviruses.

大多数人类实体瘤在临床上处于晚期时，对大多数常规疗法都有抵抗力，因此通过新策略改善预后是临床环境中的一个关键目标。我们开发了5型腺病毒，它优先在肿瘤内复制，然后检查其抗肿瘤作用。我们还对腺病毒进行了修饰，将其受体结合位点替换为35型腺病毒，由于肿瘤中受体的高表达，从而增加了对人类肿瘤的感染性。我们研究了使用感染此类腺病毒的载体细胞的可能优势。

项目成果

Cancer Gene Therapy

• DOI: 10.1177/153303460500400402
• 发表时间: 2005-02
• 期刊: Technology in Cancer Research & Treatment
• 影响因子: 2.8
• 作者: Masato Yamamoto;D. Curiel

Cytotoxicity of adenoviruses expressing the wild-type p53 gene to esophageal carcinoma cells is linked with the CAR expression level and indirectly with the endogenous p53 status

• DOI: 10.1038/cgt.2009.21
• 发表时间: 2009-11-01
• 期刊: CANCER GENE THERAPY
• 影响因子: 6.4
• 作者: Ma, G.;Kawamura, K.;Tagawa, M.
• 通讯作者: Tagawa, M.

Anti-tumor effects produced by the combinatory use of E1B-55 kDa-deleted adenoviruses and chemotherapeutic agents for human esophageal carcinoma cells.

E1B-55 kDa 缺失腺病毒与化疗药物联合使用对人食管癌细胞产生抗肿瘤作用。

• 发表时间: 2008
• 作者: Guangyu Ma;et al
• 通讯作者: et al

Combinatory cytotoxic effects produced by E1B-55kDa-deleted adenoviruses and chemotherapeutic agents are dependent on the agents in esophageal carcinoma.

• DOI: 10.1038/cgt.2010.37
• 发表时间: 2010-11
• 期刊: Cancer gene therapy
• 影响因子: 6.4

Interferon-λ induces G1 phase arrest or apoptosis in esophageal carcinoma cells and produces anti-tumor effects in combination with anti-cancer agents.

干扰素-λ可诱导食管癌细胞G1期阻滞或凋亡，并与抗癌药物联合产生抗肿瘤作用。

• 发表时间: 2010
• 期刊: Eur.J.Cancer 46
• 作者: Li Q.;Kawamura K.;Ma G.;Iwata F.;Numasaki M.;Suzuki N.;Shimada H.;Tagawa M.
• 通讯作者: Tagawa M.