the effect of vascular endothelial growth factor and hypoxia induced factor-1 to the high flow pulmonary hypertension with congenital heart disease.

血管内皮生长因子和缺氧诱导因子1对先天性心脏病高流量肺动脉高压的影响

• 批准号: 20790979
• 负责人: TOKUNAGA Chiho
• 金额: $ 1.33万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790979/
• 关键词: 肺高血圧; 血管内皮細胞増殖因子; 低酸素誘導因子; 先天性心疾患; 肺高血圧症; 高感度放射光微小血管撮影法; shearstress; ラット高血流性肺高血圧症モデル; ラット肺高血圧モデル

Congenital heart disease with high pulmonary flow is frequently complicated by the development of irreversible pulmonary hypertension. Endothelial cell injury by increased shear stress may play a key role to induce intimal and medial hypertrophy and increase vascular resistance. We analyzed the plasma VEGF (vascular endothelial growth factor) in patients with pulmonary hypertension. The plasma VEGF level in pulmonary hypertension was significantly increased compared with control (157.4±35.4pg/ml vs. 43.0±22.9pg/ml, mean±SE). Also we performed the pulmonary angiography in high flow pulmonary hypertension model rat by using synchrotron radiation micro-angiography (SRMA) to measure the pulmonary flow velocity and shear stress. The velocity of proximal pulmonary artery in pulmonary hypertension rat was 111.5±23.5mm/sec and 111.5±23.5mm/sec in control. Although, the velocity of distal pulmonary artery in pulmonary hypertension rat was significantly increased as 71.7±23.2mm/sec compared with control as 9.4±2.6mm/sec. This result suggests that higher pulmonary artery velocity may affect the endothelial cell injury and increase vascular resistance. Further evaluation about endothelial cell injury and VEGF level at the pulmonary tissue will be performed.

肺部疾病的先天性疾病有些ELL损伤可能会在肺动脉高度7.4±35.4pg/ml vs. 43.0±22.9pg的患者中起作用tole tole tole tole Tole Tole Tole Tole Tole Tole Tole Tole Tole Tole Tole Tole Tole Tole tole tole tole tole tole to tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole tole。 /ml，平均值±SE）。 mm/sec至111.5±23.5mm/sec，尽管肺中肺动物的速度显着增加，但比较71.7±23.2mm/sec，这些结果表明肺动脉速度速度可能更高CELLY并增加了对肺部组织的celly和VEGF水平的进一步评估。